公开(公告)号：US3786465A

公开(公告)日：1974-01-15

申请号：US3786465D

申请日：1972-04-17

Applicant: BECKMAN INSTRUMENTS INC

Inventor： WOODS T

IPC: G01D1/10 ,  C12M1/34 ,  C12Q1/00 ,  G01N21/27 ,  G01N21/75 ,  G01N27/00 ,  G01N27/06 ,  G01N27/416 ,  G01N33/48 ,  G01N33/483 ,  G08B21/00

CPC classification number: G01N21/272

Abstract: A rate analysis instrumentation system including a sample cell and detector for producing an electrical cell signal proportional to a parameter of a sample reacting within the cell. In a preferred embodiment the enzyme activity of a sample is measured by detecting the light absorbance characteristics of the sample. A differentiator is utilized to obtain a rate signal from the light absorbance characteristic, and the rate signal is displayed. A constant rate detection circuit is used to measure the rate signal only during periods when the reaction rate and the cell signal are linear. The constant rate detection circuit differentiates the rate signal whereby a second derivative of the cell signal is provided. The second derivative signal is compared in a comparator to a threshold value to determine when the second derivative signal approaches zero. An overrange protection circuit is provided for excluding false rate signals, such as signals having characteristics that fail to meet predetermined acceptable limits.

Abstract translation: 一种速率分析仪器系统，包括用于产生与在所述电池内反应的样品的参数成比例的电池信号的样品池和检测器。 在优选的实施方案中，通过检测样品的吸光度特性来测量样品的酶活性。 利用微分器从光吸收特性获得速率信号，并显示速率信号。 恒定速率检测电路仅在反应速率和电池信号为线性的期间用于测量速率信号。 恒定速率检测电路区分速率信号，从而提供单元信号的二阶导数。 将二进制信号在比较器中与阈值进行比较，以确定二阶导数信号何时接近零。 提供超范围保护电路用于排除诸如具有不能达到预定可接受限度的特性的信号的错误率信号。

公开(公告)号：US3786352A

公开(公告)日：1974-01-15

申请号：US3786352D

申请日：1972-04-17

Applicant: BECKMAN INSTRUMENTS INC

Inventor： WOODS T ,  RAY R

IPC: G01N27/00 ,  C12M1/34 ,  G01N21/75 ,  G01N27/416 ,  G01N33/48 ,  G01N33/483 ,  G01R1/00 ,  G01R15/08

CPC classification number: G01N27/3273

Abstract: A rate analysis instrumentation system is disclosed including a sample cell and detection means for producing an electrical cell signal proportional to a parameter of a sample reacting within the cell. In a preferred embodiment the parameter comprises the enzyme activity of a sample, measured by the light absorbance characteristics of the sample. Electrical means are provided for differentiating the electrical cell signal to obtain a rate signal, and display means are provided for displaying the rate signal or a value determined therefrom, such as enzyme activity or concentration. A constant rate detection circuit is provided to permit measurement of the rate signal only during periods when the reaction rate and the cell signal are linear. The constant rate detection circuit includes electrical means for differentiating the rate signal to derive a second derivative of the cell signal, and comparing the second derivative signal to a threshold value to determine when the second derivative signal approaches zero. Since a zero second derivative signal indicates that the rate signal is constant and the cell signal is linear, the second derivative signal is used to initiate a predetermined operation of measurement and display means in the instrument. An overrange protection circuit is also disclosed for excluding false rate signals, such as signals having characteristics that fail to meet predetermined acceptable limits. The overrange protection circuit includes means for comparing the rate signal with predetermined upper or lower amplitude limits, as well as with a total deviation limit. An overrange signal and a blank signal are produced upon departure of the rate signal from any of the predetermined limits, which signals control the measurement and display means.

Abstract translation: 公开了一种速率分析仪器系统，包括用于产生与单元内反应的样品的参数成比例的电单元信号的样品池和检测装置。 在优选实施方案中，该参数包括通过样品的吸光度特性测量的样品的酶活性。 提供电气装置用于区分电池信号以获得速率信号，并且提供显示装置用于显示速率信号或由其确定的值，例如酶活性或浓度。 提供恒定速率检测电路以仅在反应速率和电池信号为线性的时段期间测量速率信号。 恒定速率检测电路包括用于区分速率信号以导出单元信号的二阶导数的电气装置，并将二阶导数信号与阈值进行比较，以确定二阶导数信号何时接近零。 由于零二次导数信号表示速率信号恒定且单元信号是线性的，所以二阶导数信号用于启动仪器中的测量和显示装置的预定操作。 还公开了一种超范围保护电路，用于排除诸如具有不能达到预定可接受限度的特性的信号的错误率信号。 超范围保护电路包括用于将速率信号与预定的上限或下限幅度以及总偏差限制进行比较的装置。 当速率信号从任何预定限制出发时产生超范围信号和空白信号，该信号控制测量和显示装置。