公开(公告)号：US20240255516A1

公开(公告)日：2024-08-01

申请号：US18561078

申请日：2022-05-12

Applicant: CAN TECHNOLOGIES, INC.

Inventor： Jeremy Chignell ,  Cathy Staloch Hass ,  Gladys Ethel Margaria ,  Gregory Michael Poynter ,  Michael Telander ,  Stephanie Zebro ,  Alessandro Zontini

IPC: G01N33/68

CPC classification number: G01N33/6803 ,  G01N2333/962

Abstract: The present disclosure relates to in vitro methods of predicting rumen digestible protein of a feed sample. An in vitro method of predicting rumen digestible protein of a feed sample includes forming an aqueous test composition including the feed sample, a buffer, and one or more digestive enzymes. The method includes incubating the test composition. The method includes removing one or more test samples of the test composition at time points during the incubating, the time points including about 0 h and at least one time point after about 0 h. The method includes measuring soluble protein content of the one or more test samples. The method includes using the measured soluble protein content of the one or more test samples to predict a rate and/or extent of rumen protein digestion of the feed sample.