公开(公告)号：US20180251858A1

公开(公告)日：2018-09-06

申请号：US15962645

申请日：2018-04-25

Applicant: CSIR

Inventor： JEROLEN NAIDOO ,  SAMANTHA BARICHIEVY ,  RETHABILE KHUTLANG ,  MUSA M MHLANGA

IPC: C12Q1/6886 ,  A61K38/12 ,  C12Q1/70 ,  A61K31/58 ,  A61K31/7072 ,  C12N15/11 ,  A61K31/197 ,  A61K31/4709 ,  C12N15/113 ,  A61K33/24 ,  A61K31/713 ,  A61K31/675 ,  A61K31/5377 ,  A61K31/519 ,  A61K31/517 ,  A61K31/506 ,  A61K31/5025 ,  A61K31/501 ,  A61K31/496 ,  A61K31/495 ,  A61K31/475 ,  A61K31/454 ,  A61K31/4439 ,  A61K31/437 ,  A61K31/436

CPC classification number: C12Q1/6886 ,  A61K31/197 ,  A61K31/436 ,  A61K31/437 ,  A61K31/4439 ,  A61K31/454 ,  A61K31/4709 ,  A61K31/475 ,  A61K31/495 ,  A61K31/496 ,  A61K31/501 ,  A61K31/5025 ,  A61K31/506 ,  A61K31/517 ,  A61K31/519 ,  A61K31/5377 ,  A61K31/58 ,  A61K31/675 ,  A61K31/7072 ,  A61K31/713 ,  A61K33/24 ,  A61K38/12 ,  C12N15/111 ,  C12N15/1132 ,  C12N2310/113 ,  C12N2310/141 ,  C12N2320/10 ,  C12N2320/11 ,  C12N2320/30 ,  C12Q1/703 ,  C12Q2600/136 ,  C12Q2600/178

Abstract: The present invention relates to methods for the identification of anti-HIV miRNAs and anti-HIV pharmaceutical compounds using high-throughput screening methods, comprising: transfecting reporter cells with a panel of miRNAs, infecting the reporter cells with HIV, screening the cells to identify miRNAs that modulate HIV infection and identifying the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs. The invention further provides for the identification and screening of anti-HIV pharmaceutical compounds having known activity against the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs for efficacy in the treatment of HIV. The invention also provides for the use of miRNA mimics, miRNA inhibitors and pharmaceutical compounds (including oncology drugs and kinase inhibitors) in the treatment and/or prevention of HIV infection.

公开(公告)号：US20170369951A1

公开(公告)日：2017-12-28

申请号：US15694084

申请日：2017-09-01

Applicant: CSIR

Inventor： JEROLEN NAIDOO ,  SAMANTHA BARICHIEVY ,  RETHABILE KHUTLANG ,  MUSA M MHLANGA

IPC: C12Q1/68 ,  A61K31/436 ,  A61K31/437 ,  A61K31/4439 ,  A61K31/454 ,  A61K31/4709 ,  A61K31/475 ,  C12Q1/70 ,  C12N15/113 ,  C12N15/11 ,  A61K38/12 ,  A61K33/24 ,  A61K31/713 ,  A61K31/7072 ,  A61K31/675 ,  A61K31/197 ,  A61K31/5377 ,  A61K31/519 ,  A61K31/517 ,  A61K31/506 ,  A61K31/5025 ,  A61K31/501 ,  A61K31/496 ,  A61K31/495 ,  A61K31/58

CPC classification number: C12Q1/6886 ,  A61K31/197 ,  A61K31/436 ,  A61K31/437 ,  A61K31/4439 ,  A61K31/454 ,  A61K31/4709 ,  A61K31/475 ,  A61K31/495 ,  A61K31/496 ,  A61K31/501 ,  A61K31/5025 ,  A61K31/506 ,  A61K31/517 ,  A61K31/519 ,  A61K31/5377 ,  A61K31/58 ,  A61K31/675 ,  A61K31/7072 ,  A61K31/713 ,  A61K33/24 ,  A61K38/12 ,  C12N15/111 ,  C12N15/1132 ,  C12N2310/113 ,  C12N2310/141 ,  C12N2320/10 ,  C12N2320/11 ,  C12N2320/30 ,  C12Q1/703 ,  C12Q2600/136 ,  C12Q2600/178

Abstract: The present invention relates to methods for the identification of anti-HIV miRNAs and anti-HIV pharmaceutical compounds using high-throughput screening methods, comprising: transfecting reporter cells with a panel of miRNAs, infecting the reporter cells with HIV, screening the cells to identify miRNAs that modulate HIV infection and identifying the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs. The invention further provides for the identification and screening of anti-HIV pharmaceutical compounds having known activity against the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs for efficacy in the treatment of HIV. The invention also provides for the use of miRNA mimics, miRNA inhibitors and pharmaceutical compounds (including oncology drugs and kinase inhibitors) in the treatment and/or prevention of HIV infection.