公开(公告)号：US20250020639A1

公开(公告)日：2025-01-16

申请号：US18747407

申请日：2024-06-18

Applicant: CYTEK BIOSCIENCES, INC.

Inventor： Yu Rong ,  Jing Dai ,  Xingyong Wu ,  Rong Zhang ,  Peter Robles ,  Su Chen ,  Bill Godfrey ,  Ming Yan

IPC: G01N33/533 ,  C09K11/06 ,  G01N15/01 ,  G01N15/10 ,  G01N15/14 ,  G01N33/543 ,  G01N33/569

Abstract: In one embodiment, a method to synthesize highly fluorescent complexes is disclosed. The highly fluorescent complexes are synthesized by using inorganic nanoparticles, coupling agents, linkers, and fluorescent dye molecules. The unique nanoparticle-dye complexes (referred to as “SN-dye”) can provide ultra-bright fluorescent labelling. This is demonstrated by coupling the complexes to the antibody and subsequently using the conjugated antibody for more sensitive immunological detection in flow cytometry applications.