公开(公告)号：US20240376490A1

公开(公告)日：2024-11-14

申请号：US18731302

申请日：2024-06-02

Applicant: California Institute of Technology

Inventor： Yitong Ma ,  Mark W. Budde ,  Michaelle N. Mayalu ,  Michael B. Elowitz

IPC: C12N15/85 ,  C07K14/415 ,  C12N9/02 ,  C12N9/86 ,  C12N15/11

Abstract: Disclosed herein include circuits, compositions, nucleic acids, populations, systems, and methods enabling cells to sense, control, and/or respond to their own population size. In some embodiments, an orthogonal communication channel allows specific communication between engineered cells. Also described herein, in some embodiments, is an evolutionarily robust ‘paradoxical’ regulatory circuit architecture in which orthogonal signals both stimulate and inhibit net cell growth at different signal concentrations. In some embodiments, engineered cells autonomously reach designed densities and/or activate therapeutic or safety programs at specific density thresholds. Methods of treatment are also provided in some embodiments.

公开(公告)号：US11999964B2

公开(公告)日：2024-06-04

申请号：US17460120

申请日：2021-08-27

Applicant: California Institute of Technology

Inventor： Yitong Ma ,  Mark W. Budde ,  Michaelle N. Mayalu ,  Michael B. Elowitz

IPC: C12N15/85 ,  C07K14/415 ,  C12N9/02 ,  C12N9/86 ,  C12N15/11

CPC classification number: C12N15/85 ,  C07K14/415 ,  C12N9/0069 ,  C12N9/86 ,  C12N15/11 ,  C12Y113/12003 ,  C07K2319/00 ,  C12N2310/20 ,  C12N2800/107 ,  C12N2810/10

Abstract: Disclosed herein include circuits, compositions, nucleic acids, populations, systems, and methods enabling cells to sense, control, and/or respond to their own population size. In some embodiments, an orthogonal communication channel allows specific communication between engineered cells. Also described herein, in some embodiments, is an evolutionarily robust ‘paradoxical’ regulatory circuit architecture in which orthogonal signals both stimulate and inhibit net cell growth at different signal concentrations. In some embodiments, engineered cells autonomously reach designed densities and/or activate therapeutic or safety programs at specific density thresholds. Methods of treatment are also provided in some embodiments.

公开(公告)号：US20220064666A1

公开(公告)日：2022-03-03

申请号：US17460120

申请日：2021-08-27

Applicant: California Institute of Technology

Inventor： Yitong Ma ,  Mark W. Budde ,  Michaelle N. Mayalu ,  Michael B. Elowitz

IPC: C12N15/85 ,  C12N9/86 ,  C12N9/02 ,  C12N15/11 ,  C07K14/415

Abstract: Disclosed herein include circuits, compositions, nucleic acids, populations, systems, and methods enabling cells to sense, control, and/or respond to their own population size. In some embodiments, an orthogonal communication channel allows specific communication between engineered cells. Also described herein, in some embodiments, is an evolutionarily robust ‘paradoxical’ regulatory circuit architecture in which orthogonal signals both stimulate and inhibit net cell growth at different signal concentrations. In some embodiments, engineered cells autonomously reach designed densities and/or activate therapeutic or safety programs at specific density thresholds. Methods of treatment are also provided in some embodiments.