公开(公告)号：US20030104479A1

公开(公告)日：2003-06-05

申请号：US10211088

申请日：2002-08-01

Applicant: Cellomics, Inc.

Inventor： Gary Bright ,  Daniel Rajadavid Premkumar ,  Yih-Tai Chen

IPC: G01N033/53 ,  C07H021/04 ,  C12P021/04 ,  C12P021/02 ,  C12N005/06 ,  C07K016/46

CPC classification number: C12N15/62 ,  C07K2319/09 ,  C07K2319/095 ,  C07K2319/41 ,  C07K2319/60 ,  C07K2319/70

Abstract: The present invention provides novel recombinant fusion proteins for detecting binding of a molecule of interest containing a detection domain, a first and optionally a second localization domain, and a binding domain. The invention also provides recombinant nucleic acid molecules and recombinant expression vectors encoding these novel fusion proteins, genetically engineered host cells containing these expression vectors, and kits for the use of these fusion proteins, nucleic acid molecules, expression vectors, and host cells. Additionally, the present invention provides methods for identifying compounds that alter the binding of a molecule of interest in a cell.

Abstract translation: 本发明提供用于检测包含检测结构域，第一和任选的第二定位结构域和结合结构域的感兴趣分子的结合的新型重组融合蛋白。 本发明还提供重组核酸分子和编码这些新型融合蛋白的重组表达载体，含有这些表达载体的经遗传工程改造的宿主细胞，以及使用这些融合蛋白，核酸分子，表达载体和宿主细胞的试剂盒。 另外，本发明提供了鉴定化合物的方法，所述化合物改变细胞中感兴趣的分子的结合。