公开(公告)号：US09128046B2

公开(公告)日：2015-09-08

申请号：US13658263

申请日：2012-10-23

Applicant: Cellomics, Inc.

Inventor： Brent A. Samson ,  Chandrasekaran Vasudevan ,  Keith R. Heffley ,  Joseph M. Zock ,  Dirk John Vandenberg, III

IPC: G01N27/447 ,  B01L3/00 ,  B01L9/00 ,  G01N27/453

CPC classification number: G01N27/44756 ,  B01L3/50 ,  B01L3/5085 ,  B01L3/50853 ,  B01L9/00 ,  G01N27/453

Abstract: A slide holder assembly and method for use in high content screening of a comet assay. The slide holder assembly has an inside surface and is configured to receive a slide holding a plurality of biological cells on a top surface thereof. When the slide is secured within the slide holder assembly, the inside surface of the slide holder assembly and the top surface of the slide combine to form a cavity having an open top end. The cavity is watertight and configured to hold a liquid therein. A method of performing a comet assay using a slide disposed within a slide holder assembly. The method includes performing encapsulation, lysis, electrophoresis, staining, and imaging of cells on the slide while the slide remains secured within the slide holder assembly.

Abstract translation: 用于彗星测定的高含量筛选的载片架组件和方法。 滑动支架组件具有内表面，并且构造成容纳在其顶表面上容纳多个生物细胞的载片。 当滑块固定在滑块保持器组件内时，滑块保持器组件的内表面和滑块的顶表面组合形成具有开放顶端的空腔。 空腔是水密的并且构造成在其中保持液体。 使用设置在载玻片保持器组件内的滑块进行彗星测定的方法。 该方法包括在载玻片保持固定在载玻片保持器组件内时，在载玻片上进行细胞的包封，裂解，电泳，染色和成像。

公开(公告)号：US20130105320A1

公开(公告)日：2013-05-02

申请号：US13658263

申请日：2012-10-23

Applicant: Cellomics, Inc.

Inventor： Brent A. Samson ,  Chandrasekaran Vasudevan ,  Keith R. Heffley ,  Joseph M. Zock ,  Dirk John Vandenberg, III

IPC: B01L9/00 ,  G01N27/26 ,  G01N27/447 ,  G01N27/453

CPC classification number: G01N27/44756 ,  B01L3/50 ,  B01L3/5085 ,  B01L3/50853 ,  B01L9/00 ,  G01N27/453

Abstract: A slide holder assembly and method for use in high content screening of a comet assay. The slide holder assembly has an inside surface and is configured to receive a slide holding a plurality of biological cells on a top surface thereof. When the slide is secured within the slide holder assembly, the inside surface of the slide holder assembly and the top surface of the slide combine to form a cavity having an open top end. The cavity is watertight and configured to hold a liquid therein. A method of performing a comet assay using a slide disposed within a slide holder assembly. The method includes performing encapsulation, lysis, electrophoresis, staining, and imaging of cells on the slide while the slide remains secured within the slide holder assembly.

Abstract translation: 用于彗星测定的高含量筛选的载片架组件和方法。 滑动支架组件具有内表面，并且构造成容纳在其顶表面上容纳多个生物细胞的载片。 当滑块固定在滑块保持器组件内时，滑块保持器组件的内表面和滑块的顶表面组合形成具有开放顶端的空腔。 空腔是水密的并且构造成在其中保持液体。 使用设置在载玻片保持器组件内的滑块进行彗星测定的方法。 该方法包括在载玻片保持固定在载玻片保持器组件内时，在载玻片上进行细胞的包封，裂解，电泳，染色和成像。