公开(公告)号：US20040101912A1

公开(公告)日：2004-05-27

申请号：US10685737

申请日：2003-10-15

Applicant: Cellomics, Inc.

Inventor： Richard A. Rubin ,  Kenneth A. Giuliano ,  Albert H. Gough ,  R. Terry Dunlay ,  Bruce Ray Conway

IPC: G01N033/53 ,  G01N033/567 ,  G06K009/00

CPC classification number: G01N33/582 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00707 ,  B01J2219/00743 ,  B01L3/5085 ,  B82Y5/00 ,  B82Y30/00 ,  C12Q1/68 ,  C12Q1/6813 ,  C40B60/14 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5035 ,  G01N33/5076 ,  G01N33/5079 ,  G01N33/533 ,  G01N33/566 ,  G01N2015/1472 ,  G01N2015/1479 ,  G01N2015/1486 ,  G01N2015/1488 ,  G01N2015/1497 ,  G01N2035/00158 ,  G01N2035/0424 ,  G01N2333/43595 ,  G01N2333/726 ,  G06K9/0014 ,  C12Q2525/179

Abstract: The present invention provides systems, methods, and screens to measure receptor internalization in a single step with appropriate automation and throughput. This approach involves luminescent labeling of the receptor of interest and the automated measurement of receptor internalization to a perinuclear location.

Abstract translation: 本发明提供用于在单个步骤中测量受体内化的系统，方法和屏幕，具有适当的自动化和生产量。 该方法涉及感兴趣受体的发光标记和受体内化至核周位置的自动测量。

公开(公告)号：US20030204316A1

公开(公告)日：2003-10-30

申请号：US10430534

申请日：2003-05-06

Applicant: Cellomics, Inc. ,  BioDx, Inc.

Inventor： R. Terry Dunlay ,  D. Lansing Taylor

IPC: C12Q001/00 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: B82Y30/00 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00707 ,  B01J2219/00743 ,  B01L3/5085 ,  C12Q1/68 ,  C12Q1/6813 ,  C40B60/14 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/5035 ,  G01N33/582 ,  G01N2015/1486 ,  G01N2015/1497 ,  G01N2021/6419 ,  G01N2021/6482 ,  G02B21/16 ,  G02B21/365 ,  Y10S436/80 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S977/795 ,  Y10S977/881 ,  Y10S977/92 ,  Y10S977/941 ,  Y10S977/942 ,  C12Q2525/179

Abstract: The invention relates to an optical system for determining the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for specific biological activity. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a fluorescent microscope, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data

Abstract translation: 本发明涉及用于确定荧光标记的报道分子在细胞中的分布，环境或活性的光学系统，以筛选大量用于特定生物活性的化合物。 本发明涉及在荧光显微镜的阵列中提供含有荧光报告分子的细胞，并用荧光显微镜扫描各个位置的多个细胞，将光学信息转换为数字数据，并利用数字数据确定荧光的分布，环境或活性 标记的细胞中的报告分子。 位置阵列可以是工业标准96孔或384孔微量滴定板或微板，其是具有位置微距阵列中的细胞的微孔板。 本发明包括用于处理，显示和存储数据的装置和计算机化方法

公开(公告)号：US20040139103A1

公开(公告)日：2004-07-15

申请号：US10649323

申请日：2003-08-27

Applicant: Cellomics, Inc.

Inventor： Keith S. Boyce ,  Brian K. McKenna ,  Phillip W. Glick ,  R. Terry Dunlay

IPC: G06F017/00

CPC classification number: G06K9/00127 ,  G01N15/1475 ,  G01N2015/1477 ,  G01N2015/1497 ,  G01N2035/00158 ,  Y10S707/99943 ,  Y10S707/99945

Abstract: Methods and system for efficient collection and storage of experimental data. These methods and system allow experimental data from high-throughput, feature-rich data collection systems, such as high-throughput cell data collection systems to be efficiently collected, stored, managed and displayed. The methods and system can be used, for example, for storing managing and displaying cell image data and cell feature data collected from microplates including multiple wells and a variety of bio-chips in which an experimental compound has been applied to a population of cells. The methods and system provide a flexible and scalable repository of experimental data including multiple databases at multiple locations including pass-through databases that can be easily managed and allows cell data to be analyzed, manipulated and archived. The methods and system may improve the identification, selection, validation and screening of new drug compounds that have been applied to populations of cells. The methods and system can also be used to provide new bioinformatic techniques to manipulate experimental data including multiple digital photographic images.

Abstract translation: 有效收集和储存实验数据的方法和系统。 这些方法和系统允许高吞吐量，功能丰富的数据收集系统（例如高通量小区数据收集系统）的实验数据被有效地收集，存储，管理和显示。 可以使用方法和系统，例如用于存储管理和显示从包括多个孔的微孔板和其中将实验化合物应用于细胞群的各种生物芯片的微孔板收集的细胞图像数据和细胞特征数据。 这些方法和系统提供了一个灵活和可扩展的实验数据库，包括多个位置的多个数据库，包括易于管理的直通数据库，并允许细胞数据进行分析，操作和归档。 方法和系统可以改善已经应用于细胞群体的新药物的鉴定，选择，验证和筛选。 方法和系统也可用于提供新的生物信息学技术来操纵包括多个数字摄影图像的实验数据。

公开(公告)号：US20040063162A1

公开(公告)日：2004-04-01

申请号：US10686161

申请日：2003-10-15

Applicant: Cellomics, Inc.

Inventor： R. Terry Dunlay ,  D. Lansing Taylor ,  Albert H. Gough ,  Kenneth A. Giuliano

IPC: G01N033/53 ,  G01N033/567 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: B82Y30/00 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00707 ,  B01J2219/00743 ,  B01L3/5085 ,  C12Q1/68 ,  C12Q1/6813 ,  C40B60/14 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/5005 ,  G01N33/5035 ,  G01N33/582 ,  G01N2015/1486 ,  G01N2015/1497 ,  G01N2021/6419 ,  G01N2021/6482 ,  G02B21/16 ,  G02B21/365 ,  Y10S436/80 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S977/795 ,  Y10S977/881 ,  Y10S977/92 ,  Y10S977/941 ,  Y10S977/942 ,  C12Q2525/179

Abstract: The present invention provides systems, methods, and screens for an optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a high magnification fluorescence optical system, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data.

Abstract translation: 本发明提供用于细胞的光学系统分析的系统，方法和屏幕，以快速确定细胞中荧光标记的报道分子的分布，环境或活性，目的是筛选特定影响特定生物的那些化合物的大量化合物 功能。 本发明涉及在位置阵列中提供含有荧光报道分子的细胞，并用高倍放大荧光光学系统扫描每个位置中的多个细胞，将光学信息转换为数字数据，并利用数字数据确定分布，环境或活性 的荧光标记的报告分子在细胞中。 位置阵列可以是工业标准96孔或384孔微量滴定板或微板，其是具有位置微距阵列中的细胞的微孔板。 本发明包括用于处理，显示和存储数据的装置和计算机化方法。