公开(公告)号：US20230060770A1

公开(公告)日：2023-03-02

申请号：US17821120

申请日：2022-08-19

Applicant: Cephalon LLC

Inventor： Lu WANG ,  Albert KAO ,  Zhaoqing ZHANG ,  Mi JIN ,  Tianyi ZHOU

IPC: B01J20/286 ,  C07K1/22 ,  C07K16/18 ,  B01D15/36 ,  B01J20/28 ,  C12Q1/686 ,  C07K1/18 ,  C07K16/00 ,  C07K16/28

Abstract: A wash buffer comprising a surfactant for use in affinity and cation exchange chromatography to purify proteins of interest from protein aggregates and to remove and/or inactivate viruses. When used during affinity or cation exchange chromatography for the purification of a protein of interest, such as an antibody, the wash buffer significantly improves viral clearance from the preparation, while also reducing the levels of host cell proteins and protein aggregates. Following affinity or cation exchange chromatography with the wash buffer, the protein of interest may be further purified using other chromatography and filtration operations.