公开(公告)号：US12251681B2

公开(公告)日：2025-03-18

申请号：US17605502

申请日：2020-04-28

Applicant: Cytiva Bioprocess R&D AB

Inventor： Susanna Lindberg ,  Jonas Gustafsson ,  Linn Carlsson ,  Anders Hagvall ,  Erik Ringberg ,  David Jansson

IPC: B01J20/29 ,  B01J20/24 ,  B01J20/28 ,  B01J20/291 ,  B01J20/30

Abstract: The invention discloses method for manufacturing agar or agarose beads, comprising the steps of: a) providing a water phase comprising an aqueous solution of agar or agarose at a temperature of 40-100° C.: b) providing an oil phase comprising a water-immiscible solvent and an emulsifier at a temperature of 40-100° C.; c) emulsifying the water phase in the oil phase to form a water-in-oil emulsion: d) cooling the water-in-oil emulsion to a temperature below a gelation temperature of the agar or agarose to form a dispersion of solidified agar or agarose beads: and e) recovering agar or agarose beads from dispersion, wherein the emulsifier comprises a phosphate ester of an alkoxylated fatty alcohol.

公开(公告)号：US20220032266A1

公开(公告)日：2022-02-03

申请号：US17296684

申请日：2019-12-11

Applicant: Cytiva BioProcess R&D AB

Inventor： David Jansson ,  Jesper Hansson ,  Anna Akerblom ,  Emma Michaelsson

IPC: B01J20/24 ,  C08J3/075 ,  C08J3/24 ,  C12N7/00 ,  B01J20/285 ,  B01J20/06 ,  B01J20/28

Abstract: The present invention relates to porous cross-linked agarose gel beads which have a low agarose content, a method for the preparation of the beads and their use in chromatographic applications. The beads are suitable for the separation/purification of biomolecules from a biological sample. Due to the high porosity of the beads, they are especially suitable for separation/isolation of larger particles, such as virus particles e.g. adeno virus.

公开(公告)号：US20220219141A1

公开(公告)日：2022-07-14

申请号：US17605502

申请日：2020-04-28

Applicant: Cytiva Bioprocess R&D AB

Inventor： Susanna Lindberg ,  Jonas Gustafsson ,  Linn Carlsson ,  Anders Hagvall ,  Erik Ringberg ,  David Jansson

IPC: B01J20/291 ,  B01J20/24 ,  B01J20/28 ,  B01J20/30

Abstract: The invention discloses method for manufacturing agar or agarose beads, comprising the steps of: a) providing a water phase comprising an aqueous solution of agar or agarose at a temperature of 40-100° C.: b) providing an oil phase comprising a water-immiscible solvent and an emulsifier at a temperature of 40-100° C.; c) emulsifying the water phase in the oil phase to form a water-in-oil emulsion: d) cooling the water-in-oil emulsion to a temperature below a gelation temperature of the agar or agarose to form a dispersion of solidified agar or agarose beads: and c) recovering agar or agarose beads from dispersion, wherein the emulsifier comprises a phosphate ester of an alkoxy lated fatty alcohol.