公开(公告)号：US11136359B2

公开(公告)日：2021-10-05

申请号：US15900419

申请日：2018-02-20

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Tomas Bjorkman ,  Goran Bauren

IPC: C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32 ,  C07K1/22 ,  C07K14/745 ,  C07K16/00

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.