公开(公告)号：US11124539B2

公开(公告)日：2021-09-21

申请号：US16348534

申请日：2017-11-14

Applicant: Cytiva BioProcess R&D AB

Inventor： Johan William Ohman ,  Mats Ander ,  Therese Graner ,  Anneli Jorsback ,  Jinyu Zou

IPC: C07K1/22 ,  B01J20/32 ,  B01D15/38 ,  B01J20/24 ,  B01J20/285 ,  B01J20/286

Abstract: The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.