公开(公告)号：US20180306686A1

公开(公告)日：2018-10-25

申请号：US16007756

申请日：2018-06-13

Applicant: Dako Denmark A/S

Inventor： Tim Svenstrup Poulsen ,  Steen Hauge Matthiesen

IPC: G01N1/28 ,  G01N1/31 ,  G01N1/30

CPC classification number: G01N1/2813 ,  G01N1/30 ,  G01N1/312

Abstract: An apparatus (100) for processing a biological sample (101). The biological sample being arranged on a first planar surface (102) of a carrier (103). The apparatus comprises a second planar surface (104) arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply means (126, 131, 230) for supplying an amount (105) of a liquid that is to be applied to said biological sample. The first planar surface and second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said suppled amount of liquid is distributed ever said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

公开(公告)号：US20160033372A1

公开(公告)日：2016-02-04

申请号：US14883257

申请日：2015-10-14

Applicant: DAKO DENMARK A/S

Inventor： Tim Svenstrup Poulsen ,  Steen Hauge Matthiesen

IPC: G01N1/28 ,  G01N1/31

CPC classification number: G01N1/2813 ,  G01N1/30 ,  G01N1/312

Abstract: An apparatus (100) for processing a biological sample (101). The biological sample being arranged on a first planar surface (102) of a carrier (103). The apparatus comprises a second planar surface (104) arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply means (126, 131, 220) for supplying an amount (105) of a liquid that is to be applied to said biological sample. The first planar surface and the second planar surface are configured to be arranged in a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and second planar surface are brought to said second distance from each other.

Abstract translation: 一种用于处理生物样品（101）的装置（100）。 生物样品被布置在载体（103）的第一平坦表面（102）上。 该装置包括基本上平行于所述第一平面表面并且距离所述第一平面的第一距离设置的第二平表面（104），所述第二平表面以与水平面大于零度的角度（A） 生命值）; 供应装置（126,131,220），用于供应要施加到所述生物样品的液体的量（105）。 第一平坦表面和第二平坦表面被配置为彼此相距第二距离，所述第二距离使得当所述第一平面和第二平面被带入时，所述供应量的液体分布在所述生物样品上 到彼此的第二距离。

公开(公告)号：US10202638B2

公开(公告)日：2019-02-12

申请号：US15096036

申请日：2016-04-11

Applicant: Dako Denmark A/S

Inventor： Steen Hauge Matthiesen

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6841 ,  C12Q1/6832

Abstract: The invention provides methods and compositions for separately denaturing a probe and target in hybridization applications. The invention may, for example, eliminate the use of or reduce the dependence on formamide in hybridization applications. Compositions for use in the invention include an aqueous composition comprising at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.

公开(公告)号：US20140242589A1

公开(公告)日：2014-08-28

申请号：US14352815

申请日：2012-10-22

Applicant: DAKO DENMARK A/S

Inventor： Steen Hauge Matthiesen

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C12Q1/6832 ,  C12Q1/6841 ,  C12Q2523/113

Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, utilize a of cyclic and/or non-cyclic solvent that is non-toxic and may eliminate or reduce the amount of formamide in the hybridization composition.

Abstract translation: 本发明提供了用于将至少一种分子与目标物杂交的方法和组合物。 本发明可以例如利用无毒的环状和/或非环状溶剂，并且可以消除或减少杂交组合物中甲酰胺的量。

公开(公告)号：US10662465B2

公开(公告)日：2020-05-26

申请号：US14348134

申请日：2012-09-28

Applicant: DAKO DENMARK A/S

Inventor： Steen Hauge Matthiesen

IPC: C12Q1/68 ,  C12Q1/6832 ,  C12Q1/6841 ,  C12Q1/6876

Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The composition comprises at least one nucleic acid sequence, formamide, and a hybridization solution, wherein the concentration of formamide is less than or equal to 25%.

公开(公告)号：US20200056967A1

公开(公告)日：2020-02-20

申请号：US16545922

申请日：2019-08-20

Applicant: Dako Denmark A/S

Inventor： Tim Svenstrup Poulsen ,  Steen Hauge Matthiesen

IPC: G01N1/28 ,  G01N1/30 ,  G01N1/31

Abstract: An apparatus (100) for processing a biological sample (101). The biological sample being arranged on a first planar surface (102) of a carrier (103). The apparatus comprises a second planar surface (104) arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply mean (126, 131, 220) for supplying an amount (105) of a liquid that is to be applied to said biological sample. The first planar surface and said second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

公开(公告)号：US10031052B2

公开(公告)日：2018-07-24

申请号：US14883257

申请日：2015-10-14

Applicant: DAKO DENMARK A/S

Inventor： Tim Svenstrup Poulsen ,  Steen Hauge Matthiesen

IPC: G01N1/30 ,  G01N1/28 ,  G01N1/31

Abstract: An apparatus (100) for processing a biological sample (101). The biological sample being arranged on a first planar surface (102) of a carrier (103). The apparatus comprises a second planar surface (104) arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply means (126, 131, 220) for supplying an amount (105) of a liquid that is to be applied to said biological sample. The first planar surface and second planar surface are configured to be arranged in a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

公开(公告)号：US09133507B2

公开(公告)日：2015-09-15

申请号：US14105346

申请日：2013-12-13

Applicant: DAKO DENMARK A/S

Inventor： Gregory A. Testa ,  Tim Svenstrup Poulsen ,  Steen Hauge Matthiesen ,  Ole Feldballe Rasmussen ,  Lars Winther

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  G01N1/31 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12Q1/6841 ,  B01L3/508 ,  B01L7/52 ,  B01L2200/142 ,  B01L2300/043 ,  B01L2300/069 ,  B01L2300/0822 ,  B01L2300/10 ,  B01L2300/1822 ,  B01L2300/1844 ,  C12M41/14 ,  C12M41/36 ,  G01N1/312 ,  Y10T436/143333

Abstract: An apparatus for processing at least one biological sample accommodated on at least one carrier member (15) in a chamber includes, at least one reservoir (18) able to accommodate a fluid on a surface inside the chamber adjacent to and/or facing a substantial part of the at least one biological sample. The apparatus may comprise a bottom member (12) arranged to support at least one carrier member (15) carrying at least one biological sample and a lid (14) including at least one fluid reservoir (18). The reservoir filled with water provides humidity to the chamber and impedes drying out of the sample.

Abstract translation: 用于处理容纳在腔室中的至少一个载体构件（15）上的至少一个生物样品的装置包括：至少一个储存器（18），其能够容纳与所述腔室内的表面相邻和/或面对实质的流体 至少一种生物样品的一部分。 该装置可以包括底部构件（12），该底部构件（12）被布置成支撑承载至少一个生物样品的至少一个载体构件（15）和包括至少一个流体储存器（18）的盖子（14）。 充满水的储存器为室提供湿气并阻止样品干燥。

公开(公告)号：US10655177B2

公开(公告)日：2020-05-19

申请号：US14352815

申请日：2012-10-22

Applicant: DAKO DENMARK A/S

Inventor： Steen Hauge Matthiesen

IPC: C12Q1/6841 ,  C12Q1/6881 ,  C12Q1/6832

Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, utilize a of cyclic and/or non-cyclic solvent that is non-toxic and may eliminate or reduce the amount of formamide in the hybridization composition.

公开(公告)号：US10416051B2

公开(公告)日：2019-09-17

申请号：US16007756

申请日：2018-06-13

Applicant: Dako Denmark A/S

Inventor： Tim Svenstrup Poulsen ,  Steen Hauge Matthiesen

IPC: G01N1/00 ,  G01N1/28 ,  G01N1/31 ,  G01N1/30

Abstract: An apparatus (100) for processing a biological sample (101). The biological sample being arranged on a first planar surface (102) of a carrier (103). The apparatus comprises a second planar surface (104) arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply means (126, 131, 220) for supplying an amount (105) of a liquid that is to be applied to said biological sample. The first planar surface and second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.