公开(公告)号：US20210011013A1

公开(公告)日：2021-01-14

申请号：US16980724

申请日：2018-05-18

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Fang CHENG ,  Jicheng DONG ,  Bingbing SUN ,  Huanan WANG

IPC: G01N33/543 ,  C12Q1/6837 ,  C07C317/18 ,  C07C315/04 ,  G01N21/64

Abstract: The present disclosure discloses a novel biochip substrate, a preparation method and an application thereof. The surface of the novel biochip substrate contains active vinyl sulfone groups. The preparation method involves a one-step reaction of a compound containing vinyl sulfone groups on both ends with a silicon-hydroxyl group on the surface of a silicon-based biochip substrate material under catalytic conditions, to prepare the biochip substrate. The application immobilizes biomacromolecules by conducting Michael addition of amino or sulfydryl group in biomacromolecules and the vinyl sulfone group on the surface of the biochip substrate, realizing biological functionalization thereof. The biochip substrate has high-density active vinyl sulfone groups, which can be used for immobilization of various biomolecules with mild fixation conditions and simple operation. The preparation method does not require complex pretreatment processes, and has high operability and reproducibility, low cost, mild reaction conditions, simple operation, and environmentally friendly, which is a broad-spectrum biochip substrate with great potential.

公开(公告)号：US20200238254A1

公开(公告)日：2020-07-30

申请号：US16486765

申请日：2018-05-18

Applicant: Dalian University of Technology

Inventor： Fang CHENG ,  Mingyang LI ,  Wei HE ,  Bingbing SUN ,  Xiaochun MA ,  Jingping QU

IPC: B01J20/26 ,  B01J20/28 ,  C07K1/22

Abstract: In a method to functionalize high-density chromatography matrix functionalization method, the hydrophilic microsphere having a surface polyhydroxy structure or the hydrophilic microsphere coated with a polyhydroxy polymer is used as chromatography matrix, under anhydrous conditions with catalyst, catalyze surface hydroxyl group of the matrix to react with divinyl sulfone, and achieve functionalization of the hydroxyl group on the surface of the matrix. The chromatography matrix surface functionalization method of the present disclosure has few steps, simple process and high functional density, and the reagents and solvents used are conventional reagents and can be recycled and reused. No byproduct is generated during the reaction with high atom economy and low cost, and the density control can be achieved by adjusting the reaction time and catalyst type.

公开(公告)号：US20230241209A1

公开(公告)日：2023-08-03

申请号：US18002822

申请日：2021-12-20

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Changying XUE ,  Bingbing SUN ,  Hang BAO ,  Zhihui LIANG

IPC: A61K39/39

CPC classification number: A61K39/39 ,  A61K2039/55505

Abstract: A method for preparing a combination adjuvant is based on carboxyl modified aluminum oxyhydroxide nanoparticles. The preparation method uses carboxylated hydroxyl oxide nanoparticles as a carrier, but is not limited to the role of a carrier. The carboxylated hydroxyl oxide nanoparticles are combined with a novel CpG-ODN adjuvant, such that the half-life period of a CpG adjuvant is prolonged. The combination of adjuvants shows a synergistic effect, such that the Th2 type immune stimulation ability is enhanced, and the possibility of Th1 type immunity is also given to the adjuvant.

公开(公告)号：US20240342276A1

公开(公告)日：2024-10-17

申请号：US18293608

申请日：2022-11-25

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Bingbing SUN ,  Min LI ,  Changying XUE ,  Zhihui LIANG ,  Lei ZHANG ,  Chen CHEN

IPC: A61K39/39 ,  A61K39/00 ,  A61K39/12 ,  A61K39/29 ,  A61P37/04

CPC classification number: A61K39/39 ,  A61K39/12 ,  A61K39/292 ,  A61P37/04 ,  A61K2039/5258 ,  A61K2039/55505

Abstract: A silicon dioxide vaccine delivery system uses virus-like particles as templates. The particle morphology of the silicon dioxide vaccine system is 50-500 nm of nanoparticles, of which an antigenic component is 20-200 nm of virus-like particles, an adjuvant component is nano silicon dioxide, the silicon dioxide component is wrapped on the surface of the virus-like particle, and a mass ratio of silicon element to antigen is 50-0.5:1. The construction of the silicon dioxide vaccine delivery system includes steps of: (1) adding a proper amount of 3-aminopropyltriethoxysilane into an aqueous solution containing virus-like particles and stirring; (2) adding a proper amount of tetraethoxysilane into the dispersion system in step (1) and stirring; and (3) centrifuging a reactant obtained in step (2) and removing a supernatant to obtain a product. A vaccine constructed by means of the vaccine system can trigger a host to generate humoral and cellular immune levels.