公开(公告)号：US20230167473A1

公开(公告)日：2023-06-01

申请号：US17780423

申请日：2021-11-17

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Chuang XUE ,  Mingming QI ,  Wenqiang ZHANG

IPC: C12P17/06 ,  C12N15/52 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P17/06 ,  C12N15/52 ,  C12N9/0004 ,  C12Y121/99 ,  C12N9/0006 ,  C12Y101/01157 ,  C12N9/001 ,  C12Y103/01044 ,  C12N9/1029 ,  C12Y203/01009 ,  C12N9/88 ,  C12Y402/01017 ,  C12Y404/01026 ,  C12N9/93 ,  C12Y604/01002

Abstract: A recombinant host cell capable of biosynthesizing cannabichromenic acid and a construction method thereof, and a method for biosynthesizing cannabichromenic acid through the recombinant host cell. Saccharomyces cerevisiae is taken as a host. First, cannabigerolic acid synthase and cannabichromenic acid synthase are over-expressed in the host; then, a metabolic pathway of a precursor compound, olivetolic acid, synthesizing cannabichromenic acid from saccharides is constructed in the host, a metabolic pathway for hexanoic acid to olivetolic acid is further constructed in the host, an endogenous mevalonate pathway of the host and a metabolic pathway of acetyl-CoA are optimized, cannabichromenic acid synthase is rationally designed, highly active cannabichromenic acid synthase is screened out, and finally, a cannabichromene pathway is located to peroxisomes and lipid droplets by using the cell compartmentalization principle to obtain recombinant Saccharomyces cerevisiae capable of biosynthesizing cannabichromenic acid.