公开(公告)号：US20210088486A1

公开(公告)日：2021-03-25

申请号：US16976751

申请日：2019-01-29

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Jason Joshua COURNOYER ,  Scott B. DANIELS ,  Aaron James HUDSON ,  Subhasish PURKAYASTHA

IPC: G01N30/86 ,  G01N30/14 ,  G01N30/72 ,  G01N30/88 ,  G01N30/60 ,  G01N1/40

Abstract: A method and kit for detecting at least two vitamin D metabolites in a biological sample is disclosed, which comprises processing the biological sample to prepare the sample for LC-MS/MS analysis, passing the prepared sample through a liquid chromatography column having an outlet connected to an inlet port of a tandem mass spectrometer to separate said two vitamin D metabolites, if present in the sample, and introduce the two vitamin D metabolites into the tandem mass spectrometer. The method further comprises generating [M+H]+ ions of each of the two vitamin D metabolites in said tandem mass spectrometer, and generating two fragment ions of said [M+H]+ ions associated with said vitamin D metabolites, wherein said fragment ions are not due to water losses from the [M+H]+ ions; and detecting the fragment ions to identify presence of the two metabolites in the biological sample.