公开(公告)号：US10301676B2

公开(公告)日：2019-05-28

申请号：US15611917

申请日：2017-06-02

Applicant: Direct Genomics Co., Ltd.

Inventor： Qin Yan ,  Zefei Jiang ,  Jiao Zheng ,  Ping Wu ,  Zhiliang Zhou ,  Liangjin Ge

IPC: G01N21/00 ,  C12Q1/6869 ,  C12M1/34 ,  B01L3/00 ,  B01L9/00 ,  G01N21/64 ,  G02B21/06 ,  G02B21/16 ,  G02B21/24

Abstract: A nucleic acid sequencing system is provided, including a base provided with a clamping platform, a reagent storage unit, a fluid control unit, a mobile platform and a total reflection microscope thereon; the clamping platform being provided with a gene sequencing chip thereon; the reagent storage unit being configured to store a gene sequencing reagent, the fluid control unit being configured to pump the gene sequencing reagent from the reagent storage unit to the gene sequencing chip, the mobile platform being configured to drive the clamping platform to move toward or away from the total reflection microscope; and the total reflection microscope being configured to detect a gene sequence of a sample in the gene sequencing chip.

公开(公告)号：US20190001327A1

公开(公告)日：2019-01-03

申请号：US15752412

申请日：2016-08-12

Applicant: DIRECT GENOMICS CO., LTD.

Inventor： Ping Wu ,  Qin Yan

IPC: B01L3/00 ,  C12Q1/6837

CPC classification number: B01L3/502707 ,  B01L3/5027 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/166 ,  C12M1/00 ,  C12M1/34 ,  C12Q1/68 ,  C12Q1/6837

Abstract: Disclosed is a chip. The chip comprises a substrate (1) and a base layer (2) in pressing arrangement with the substrate; the substrate comprises a first surface (1a) and a second surface (1b) in opposite arrangement, reaction tank arrays formed by a plurality of flowing channels (11) are arranged on the first surface of the substrate at intervals, two oppositely arranged side walls (111, 112) of each flowing channel (11) stretch along the length direction of the flowing channel (11) and intersect at two ends of the flowing channel to form two tapered tail ends (113) with included angles, and a fluid inlet hole (12) and a fluid outlet hole (13) which are communicated with the second surface of the substrate are respectively provided on the surfaces of the two tapered tail ends (113); and the base layer (2) comprises a transparent base (21) and a spacing layer (22) arranged on the surface of the transparent base, the spacing layer (22) is in contact with the firs surface (1a) of the substrate, and a corrosion groove is provided on the spacing layer (22) corresponding to a position where the flowing channel (11) is located. The flow field distribution of the chip is good, the deformation rate of a base in the chip is low, and the fluid in the chip can be fully flushed or replaced. Also disclosed is an application of the chip.

公开(公告)号：US20190011365A1

公开(公告)日：2019-01-10

申请号：US15752417

申请日：2016-09-06

Applicant: DIRECT GENOMICS CO., LTD.

Inventor： Liangjin Ge ,  Luyang Zhao ,  Ping Wu ,  Qin Yan

IPC: G01N21/64 ,  G02B21/18 ,  G02B21/16 ,  G02B21/06

CPC classification number: G01N21/6452 ,  G01N21/6458 ,  G01N21/648 ,  G01N2021/6419 ,  G02B21/0076 ,  G02B21/06 ,  G02B21/16 ,  G02B21/18 ,  G02B21/245 ,  G02B27/141

Abstract: The present disclosure discloses a total internal reflection fluorescence imaging system and a sequencing device. The total internal reflection fluorescence imaging system includes a first imaging system and a second imaging system. The first imaging system includes N separate laser light paths. The second imaging system determines the changes of the height of the sample by means of the differences of the positions at which the second receiving device acquires the second images, and performs automatic compensation according to the changes of the height of the sample, so as to ensure that the sample is always at a focused position. The embodiment can perform light path adjustment of the N separate laser light paths respectively by means of the second imaging system, so as to realize that the N separate laser light paths have the same penetration depth. Therefore no further light path adjustment is required when switching between different light sources, thus saving the time consuming for light path adjustment.