公开(公告)号：US20140350868A1

公开(公告)日：2014-11-27

申请号：US14359606

申请日：2012-11-13

Applicant: EPISENTEC AB

Inventor： Anders Hanning

IPC: G01N21/59

CPC classification number: G01N21/59 ,  G01N21/274 ,  G01N21/553 ,  G01N2021/5903 ,  G01N2201/129

Abstract: The present invention provides a method of determining the amount of an optical probe species binding to or releasing from an optical sensor surface characterized in that the determination comprises the steps of: a) determining, at one single wavelength or at more than one wavelength, a physical measurand (xi) that is related to the absorptivity of said probe, b) correlating the value of the measurand to the amount of said optical probe species binding to or releasing from said surface, respectively, wherein the physical measurand (xi) of step a) is a physical measurand in which the contribution from the refractive index is substantially zero. The present invention further provides different uses of a peak width as well as a computer program product and reagent kits for the disclosed methods.

Abstract translation: 本发明提供一种确定与光学传感器表面结合或从光学传感器表面释放的光学探针物质的量的方法，其特征在于，所述测定包括以下步骤：a）在单个波长或多于一个波长处确定 与所述探针的吸收性相关的物理测量（xi），b）分别将所述被测量的值与所述光学探针物质与所述表面结合或释放的量相关联，其中所述步骤的物理测量（xi） a）是物理测量，其中折射率的贡献基本为零。 本发明进一步提供峰宽度的不同用途以及用于所公开方法的计算机程序产品和试剂盒。