公开(公告)号：US20150225750A1

公开(公告)日：2015-08-13

申请号：US14422180

申请日：2013-08-14

Applicant: Easel Biotechnologies, LLC

Inventor： Yi-Xin Huo ,  Kwang Myung Cho

IPC: C12P7/16 ,  C12M1/40 ,  C12M1/00

CPC classification number: C12P7/16 ,  C12M21/12 ,  C12M21/18 ,  C12M23/58 ,  C12P7/56 ,  C12P13/06 ,  C12P13/08 ,  C12P39/00 ,  C12Y101/01001 ,  C12Y104/01009 ,  Y02E50/10

Abstract: Methods and systems for the production of alcohols are described. A two stage process is utilized, where fermentation in a first stage produces an intermediate product, such as an amino acid or organic acid, from a carbon containing feedstock. A second stage produces alcohol by fermentation of this intermediate product.

Abstract translation: 描述了用于生产醇的方法和系统。 利用两阶段方法，其中第一阶段的发酵从含碳原料产生中间产物，例如氨基酸或有机酸。 第二阶段通过该中间产物的发酵产生醇。

公开(公告)号：US10385366B2

公开(公告)日：2019-08-20

申请号：US16157299

申请日：2018-10-11

Applicant: Easel Biotechnologies, LLC

Inventor： Yi-Xin Huo ,  Lars Erik Ulf Rohlin ,  Shahrooz Rabizadeh

IPC: C12P7/40 ,  C12P7/24 ,  C12P7/06 ,  C12P7/02

Abstract: Systems, methods, and metabolically engineered microorganisms are contemplated in which low molecular weight gases, and especially methane and syngas are used as a carbon source, and in which CO2 or formaldehyde and reduction equivalents are generated for use in in vivo production of the value products.

公开(公告)号：US10106825B1

公开(公告)日：2018-10-23

申请号：US15336744

申请日：2016-10-27

Applicant: Easel Biotechnologies, LLC

Inventor： Yi-Xin Huo ,  Lars Erik Ulf Rohlin ,  Shahrooz Rabizadeh

IPC: C12P7/16 ,  C12P7/06 ,  C12N15/52 ,  C12P7/40 ,  C12P7/02 ,  C12P7/24

Abstract: Systems, methods, and metabolically engineered microorganisms are contemplated in which low molecular weight gases, and especially methane and syngas are used as a carbon source, and in which CO2 or formaldehyde and reduction equivalents are generated for use in in vivo production of the value products.

公开(公告)号：US10676762B2

公开(公告)日：2020-06-09

申请号：US16264009

申请日：2019-01-31

Applicant: Easel Biotechnologies, LLC

Inventor： Yi-Xin Huo ,  Kwang Myung Cho

IPC: C12N1/00 ,  C12N1/10 ,  C12P7/64 ,  C12P7/16 ,  C12P39/00 ,  C12M1/40 ,  C12M1/00 ,  C12P13/06 ,  C12P7/56 ,  C12P13/08

Abstract: Methods and systems for the production of alcohols are described. A two stage process is utilized, where fermentation in a first stage produces an intermediate product, such as an amino acid or organic acid, from a carbon containing feedstock. A second stage produces alcohol by fermentation of this intermediate product.

公开(公告)号：US20190040421A1

公开(公告)日：2019-02-07

申请号：US16157299

申请日：2018-10-11

Applicant: Easel Biotechnologies, LLC

Inventor： Yi-Xin Huo ,  Lars Erik Ulf Rohlin ,  Shahrooz Rabizadeh

IPC: C12P7/40 ,  C12P7/02 ,  C12P7/24 ,  C12P7/06

CPC classification number: C12P7/40 ,  C12P7/02 ,  C12P7/065 ,  C12P7/24 ,  Y02E50/17

Abstract: Systems, methods, and metabolically engineered microorganisms are contemplated in which low molecular weight gases, and especially methane and syngas are used as a carbon source, and in which CO2 or formaldehyde and reduction equivalents are generated for use in in vivo production of the value products.

公开(公告)号：US10113160B2

公开(公告)日：2018-10-30

申请号：US15115670

申请日：2015-01-30

Applicant: Easel Biotechnologies, LLC

Inventor： Yi-Xin Huo ,  Benjamin Schilling ,  Shahrooz Rabizadeh

IPC: C12P7/16 ,  C12P7/62 ,  C12P5/02 ,  C12N9/88 ,  C12N9/12 ,  C10L1/00 ,  C12N15/74

Abstract: Genetically engineered cells and methods are presented that allow for the production of various value products from CO2. Contemplated cells have a CBB cycle that is genetically modified such that two molecules of CO2 fixed in the CBB cycle can be withdrawn from the modified CBB cycle as a single C2 compound. In contemplated aspects a CBB cycle includes an enzymatic activity that generates the single C2 compound from a compound of the CBB cycle, while further modifications to the CBB cycle will not introduce additional recombinant enzymatic activity/activities outside the already existing catalytic activities in the CBB cycle.

公开(公告)号：US20180216096A1

公开(公告)日：2018-08-02

申请号：US15938380

申请日：2018-03-28

Applicant: Easel Biotechnologies, LLC

Inventor： Yi-Xin Huo ,  Benjamin Schilling ,  Shahrooz Rabizadeh

IPC: C12N9/88 ,  C12N9/12 ,  C12P7/62 ,  C12P7/16 ,  C10L1/00 ,  C12N15/74 ,  C12P5/02

CPC classification number: C12N9/88 ,  C10L1/00 ,  C10L2200/0469 ,  C10L2290/26 ,  C12N1/20 ,  C12N9/1205 ,  C12N15/74 ,  C12P5/026 ,  C12P7/16 ,  C12P7/625 ,  C12Y207/01019 ,  C12Y401/02009 ,  Y02E50/10

Abstract: Genetically engineered cells and methods are presented that allow for the production of various value products from CO2. Contemplated cells have a CBB cycle that is genetically modified such that two molecules of CO2 fixed in the CBB cycle can be withdrawn from the modified CBB cycle as a single C2 compound. In contemplated aspects a CBB cycle includes an enzymatic activity that generates the single C2 compound from a compound of the CBB cycle, while further modifications to the CBB cycle will not introduce additional recombinant enzymatic activity/activities outside the already existing catalytic activities in the CBB cycle.

公开(公告)号：US20160348086A1

公开(公告)日：2016-12-01

申请号：US15115670

申请日：2015-01-30

Applicant: EASEL BIOTECHNOLOGIES, LLC

Inventor： Yi-Xin Huo ,  Benjamin Schilling ,  Shahrooz Rabizadeh

IPC: C12N9/88 ,  C12P5/02 ,  C12N15/74 ,  C12N9/12 ,  C10L1/00 ,  C12P7/16

CPC classification number: C12N9/88 ,  C10L1/00 ,  C10L2200/0469 ,  C10L2290/26 ,  C12N9/1205 ,  C12N15/74 ,  C12P5/026 ,  C12P7/16 ,  C12P7/625 ,  C12Y207/01019 ,  C12Y401/02009 ,  Y02E50/10

Abstract: Genetically engineered cells and methods are presented that allow for the production of various value products from CO2. Contemplated cells have a CBB cycle that is genetically modified such that two molecules of CO2 fixed in the CBB cycle can be withdrawn from the modified CBB cycle as a single C2 compound. In contemplated aspects a CBB cycle includes an enzymatic activity that generates the single C2 compound from a compound of the CBB cycle, while further modifications to the CBB cycle will not introduce additional recombinant enzymatic activity/activities outside the already existing catalytic activities in the CBB cycle.

Abstract translation: 提出了基因工程细胞和方法，允许从二氧化碳生产各种价值产品。 考虑的细胞具有遗传修饰的CBB循环，使得在CBB循环中固定的两个分子的CO 2可以作为单一的C2化合物从经修饰的CBB循环中排出。 在预期的方面，CBB循环包括从CBB循环的化合物产生单一C2化合物的酶活性，而对CBB循环的进一步修饰将不会在CBB循环中已经存在的催化活性之外引入另外的重组酶活性/活性 。

公开(公告)号：US10385328B2

公开(公告)日：2019-08-20

申请号：US15938380

申请日：2018-03-28

Applicant: Easel Biotechnologies, LLC

Inventor： Yi-Xin Huo ,  Benjamin Schilling ,  Shahrooz Rabizadeh

IPC: C12N1/20 ,  C12N9/88 ,  C12N9/12 ,  C12P7/16 ,  C10L1/00 ,  C12N15/74 ,  C12P7/62 ,  C12P5/02

Abstract: Genetically engineered cells and methods are presented that allow for the production of various value products from CO2. Contemplated cells have a CBB cycle that is genetically modified such that two molecules of CO2 fixed in the CBB cycle can be withdrawn from the modified CBB cycle as a single C2 compound. In contemplated aspects a CBB cycle includes an enzymatic activity that generates the single C2 compound from a compound of the CBB cycle, while further modifications to the CBB cycle will not introduce additional recombinant enzymatic activity/activities outside the already existing catalytic activities in the CBB cycle.

公开(公告)号：US10233467B2

公开(公告)日：2019-03-19

申请号：US14422180

申请日：2013-08-14

Applicant: Easel Biotechnologies, LLC

Inventor： Yi-Xin Huo ,  Kwang Myung Cho

IPC: C12P7/64 ,  C12P7/16 ,  C12P7/56 ,  C12N1/00 ,  C12N9/10 ,  C12M1/40 ,  C12M1/00 ,  C12P39/00 ,  C12P13/08 ,  C12P13/06

Abstract: Methods and systems for the production of alcohols are described. A two stage process is utilized, where fermentation in a first stage produces an intermediate product, such as an amino acid or organic acid, from a carbon containing feedstock. A second stage produces alcohol by fermentation of this intermediate product.