公开(公告)号：US10961515B1

公开(公告)日：2021-03-30

申请号：US16904450

申请日：2020-06-17

Applicant: Fudan University

Inventor： Fener Chen ,  Zedu Huang ,  Zexu Wang ,  Minjie Liu

IPC: C12N9/04 ,  C12N1/20 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12P7/62

Abstract: Disclosed herein are a carbonyl reductase variant and its use in the preparation of (R)-4-chloro-3-hydroxybutyrate. The carbonyl reductase variant is obtained by mutating phenylalanine-85 in an amino acid sequence as shown in SEQ ID NO:4 to methionine. An amino acid or amino acids at one or more positions other than position 85 in the amino acid sequence of the carbonyl reductase may be further replaced. The application also provides a recombinant expression vector carrying the gene encoding the carbonyl reductase variant, a genetically-engineered bacterium carrying the carbonyl reductase variant gene and glucose dehydrogenase gene, and an application of this bacterium in the asymmetric reduction of 4-chloroacetoacetate to prepare (R)-4-chloro-3-hydroxybutyrate.

公开(公告)号：US11555008B2

公开(公告)日：2023-01-17

申请号：US17123098

申请日：2020-12-15

Applicant: Fudan University

Inventor： Fener Chen ,  Dang Cheng ,  Minjie Liu ,  Meifen Jiang ,  Zedu Huang ,  Zexu Wang ,  Jiaqi Wang

IPC: B01J19/00 ,  C07C227/04 ,  C07C229/22

Abstract: A method for preparing L-carnitine using a micro-reaction system. (R)-4-halo-3-hydroxybutyrate was subjected to quaternization and hydrolysis in an aqueous trimethylamine solution in the presence of an inorganic base in a micro-channel reactor to produce the L-carnitine.

公开(公告)号：US10526622B2

公开(公告)日：2020-01-07

申请号：US15871039

申请日：2018-01-14

Applicant: FUDAN UNIVERSITY

Inventor： Fener Chen ,  Zedu Huang ,  Ge Meng ,  Minjie Liu ,  Zhining Li ,  Zexu Wang ,  Haihui Peng ,  Fangjun Xiong ,  Yan Wu ,  Yuan Tao

IPC: C12P7/62 ,  C07C69/732 ,  C12N9/04 ,  C07C67/28 ,  G01N30/02

Abstract: The present disclosure relates to the technical field of biochemical engineering and particularly discloses a preparation method for (R)-3-hydroxyl-5-hexenoate. In the method of the present disclosure, the (R)-3-hydroxyl-5-hexenoate is prepared by catalytic reduction of 3-carbonyl-5-hexenoate by ketoreductase with 3-carbonyl-5-hexenoate as the substrate. The amino acid sequence of ketoreductase is shown in SEQ ID NO.1. In the present disclosure, the (R)-3-hydroxyl-5-hexenoate having a very high chiral purity is obtained by asymmetric reduction by ketoreductase as the biocatalyst. The present disclosure has the advantages of easy operation, mild reaction conditions, high reaction yield and good practical industrial application value.