公开(公告)号：US20150040467A1

公开(公告)日：2015-02-12

申请号：US14521618

申请日：2014-10-23

Applicant: FUJIFILM CORPORATION

Inventor： Hideyuki KANEHARA ,  Tadashi MATSUNAGA ,  Tsuyoshi TANAKA ,  Masayoshi TANAKA

IPC: C12N1/12 ,  C10L1/00 ,  C10L1/02 ,  C12P7/64

CPC classification number: C12N1/12 ,  C10L1/00 ,  C10L1/02 ,  C10L2200/0469 ,  C12P7/64 ,  C12P7/6463 ,  C12R1/89 ,  Y02P20/582

Abstract: There is provided a method for culturing microalgae in which microalgae capable of forming a biofilm on a liquid surface are cultured so as to form a biofilm on a liquid surface of a liquid medium, and microalgae capable of forming the biofilm on the liquid surface, for example, microalgae closely related to Botryococcus sudeticus.

Abstract translation: 提供了一种培养微藻的方法，其中培养能够在液体表面上形成生物膜的微藻，以便在液体介质的液体表面上形成生物膜，以及能够在液体表面上形成生物膜的微藻，用于 例如，与Botryococcus sudeticus密切相关的微藻。

公开(公告)号：US20150267162A1

公开(公告)日：2015-09-24

申请号：US14733140

申请日：2015-06-08

Applicant: FUJIFILM Corporation

Inventor： Hideyuki KANEHARA ,  Tadashi MATSUNAGA ,  Tsuyoshi TANAKA ,  Masayoshi TANAKA

IPC: C12N1/12 ,  C12P7/64

CPC classification number: C12N1/12 ,  A01G33/00 ,  C12P7/6409 ,  Y02A40/88 ,  Y02P20/582

Abstract: There is provided a method of culturing microalgae, including: culturing in a first stage in which microalgae obtained through a purification process are cultured in a culture solution within a first culture container to form a biofilm as a film-like structure or a three-dimensional structure which is formed of the microalgae on a liquid surface of the culture solution; and culturing in a second stage in which microalgae are cultured on a liquid surface using at least a part of the biofilm as a film-like structure or a three-dimensional structure which is formed on the liquid surface of the culture solution, as seed algae.

Abstract translation: 提供了一种培养微藻的方法，包括：在第一阶段培养，其中通过纯化过程获得的微藻在第一培养容器内的培养溶液中培养以形成膜状结构的生物膜或三维 在培养液的液面上由微藻形成的结构; 并且在使用至少一部分生物膜作为膜状结构或形成在培养液的液面上的三维结构的液体表面上培养微藻的第二阶段中进行培养，作为种子藻 。

公开(公告)号：US20160194671A1

公开(公告)日：2016-07-07

申请号：US15074928

申请日：2016-03-18

Applicant: FUJIFILM Corporation

Inventor： Hideyuki KANEHARA ,  Tadashi MATSUNAGA ,  Tsuyoshi TANAKA ,  Masayoshi TANAKA ,  Masaki MUTO

IPC: C12P7/64 ,  C12R1/89 ,  C12N1/12

CPC classification number: C12P7/64 ,  C12N1/12 ,  C12P7/6463 ,  C12R1/89 ,  Y02P20/582

Abstract: In a liquid surface-floating culture method in which culturing of microalgae is performed on the liquid surface, there is provided a culture method in which the oil content in microalgae is improved. In addition, there is a provided a method for decreasing the probability of recovery of bottom surface algae. Furthermore, an object of the present invention is to provide a culture method in which the proliferation rate of microalgae is improved.Culturing is performed such that a medium is suctioned from a region, in which there is a small quantity of microalgae between the liquid surface and the bottom surface, is discarded, and is replaced with a medium of which the concentration of a nitrogen compound or a phosphorus compound is lower than that of the above-described medium. In addition, a liquid is added thereto immediately before collecting microalgae on the liquid surface, and the water depth in a culture vessel is made to be deep. In addition, the microalgae are subjected to liquid surface-floating culture using a medium containing sugar. In the present invention, microalgae which can form a biofilm on the liquid surface and have at least one characteristic selected from the group consisting of the following (1) to (8) when being cultured in a medium within a culture vessel may be used. (1) The sum of the quantity of algal bodies of microalgae existing on the liquid surface and in a region from 1 cm below the liquid surface to the liquid surface, and the quantity of algal bodies of microalgae on the bottom surface of a culture vessel is greater than or equal to 10 times the quantity of algal bodies existing in the other region within the culture vessel; (2) the specific gravity of microalgae on the liquid surface is smaller than that of microalgae on the bottom surface of the culture vessel; (3) the specific gravity of microalgae on the liquid surface is greater than that of water; (4) the oil content of microalgae on the liquid surface is higher than that of microalgae on the bottom surface; (5) the size of microalgae on the liquid surface is larger than that of microalgae on the bottom surface; (6) a formed biofilm includes a film-like outer layer and an inner layer which has a plurality of bubble-like structures, and the outer layer is thicker than the inner layer; (7) a part of a formed biofilm has a pleat-like structure in a medium; and (8) in a case where microalgae obtained by collecting a biofilm which has been formed and subjecting the collected biofilm to suspension treatment are seeded on the liquid surface of a medium, the microalgae can be deposited in the medium.

公开(公告)号：US20160194600A1

公开(公告)日：2016-07-07

申请号：US15074966

申请日：2016-03-18

Applicant: FUJIFILM Corporation

Inventor： Hideyuki KANEHARA ,  Tadashi MATSUNAGA ,  Tsuyoshi TANAKA ,  Masayoshi TANAKA ,  Masaki MUTO

IPC: C12N1/12 ,  C12P7/64

CPC classification number: C12N1/12 ,  C12N1/02 ,  C12P7/64 ,  C12R1/89 ,  Y02P20/582

Abstract: An object of the present invention is to provide a method for performing fluid surface-floating culture of microalgae without supplying new seed algae. Microalgae on the bottom surface are used as seed algae. That is, there is provided a method for culturing microalgae which includes: a first culture process of culturing microalgae in a medium within a culture vessel, forming a biofilm on the liquid surface of the medium, and maintaining the microalgae on the bottom surface of the culture vessel; a process of collecting at least a part of the biofilm on the liquid surface formed in the first culture process and leaving at least some microalgae on the bottom surface inside the culture vessel; and a second culture process of culturing the microalgae remaining on the bottom surface within the identical culture vessel, and forming a biofilm on the liquid surface of the medium. In addition, the present invention provides novel microalgae which can form a biofilm on the liquid surface.

Abstract translation: 本发明的目的是提供一种在不提供新的种子藻的情况下进行微藻的流体表面漂浮培养的方法。 底面的微藻用作种子藻类。 也就是说，提供了一种培养微藻的方法，其包括：在培养容器内的培养基中培养微藻的第一培养方法，在培养基的液体表面上形成生物膜，并将微藻保持在培养皿的底面上 文化船 在第一培养过程中形成的液体表面上收集至少一部分生物膜并在培养容器内的底表面上留下至少一些微藻的过程; 以及培养残留在同一培养容器内的底面上的微藻的第二培养方法，在培养基的液面上形成生物膜。 此外，本发明提供了可以在液体表面上形成生物膜的新型微藻。