公开(公告)号：US11913055B2

公开(公告)日：2024-02-27

申请号：US17504858

申请日：2021-10-19

Applicant: Fudan University

Inventor： Fener Chen ,  Dang Cheng ,  Zedu Huang ,  Chen Hu ,  Meifen Jiang ,  Minjie Liu ,  Huashan Huang

IPC: C12P7/42 ,  C12P7/62 ,  C12N9/04 ,  C12M3/06 ,  C12N11/084 ,  C12N11/089 ,  C12M1/40 ,  C12M1/00

CPC classification number: C12P7/62 ,  C12M21/18 ,  C12M23/16 ,  C12M29/00 ,  C12N11/084 ,  C12N11/089 ,  C12Y101/0108 ,  C12Y101/01184

Abstract: Disclosed herein relates to biopharmaceuticals, and more particularly to a continuous flow method for preparing (R)-3-hydroxy-5-hexenoate. Carbonyl reductase and isopropanol dehydrogenase are co-immobilized onto an inert solid medium simultaneously to prepare a carbonyl reductase/isopropanol dehydrogenase co-immobilized catalyst, which is then filled into a microchannel reactor of the micro reaction system. A solution containing substrate 3-carbonyl-5-hexenoate is subsequently pumped into the microchannel reactor to perform an asymmetric carbonyl reduction reaction to obtain (R)-3-hydroxy-5-hexenoate.