公开(公告)号：US20180244729A1

公开(公告)日：2018-08-30

申请号：US15900419

申请日：2018-02-20

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Tomas BJORKMAN ,  Goran Bauren

IPC: C07K14/31 ,  B01D15/38 ,  C07K14/745 ,  C07K1/22 ,  C07K16/00 ,  B01J20/32 ,  B01J20/286

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/745 ,  C07K16/00

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.

公开(公告)号：US10189891B2

公开(公告)日：2019-01-29

申请号：US14385336

申请日：2013-03-26

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Mats Ander ,  Goran Bauren ,  Tomas Bjorkman ,  Per-Mikael Aberg ,  Gustav Rodrigo

IPC: C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  B01J20/286 ,  B01D15/38 ,  B01J20/291 ,  G01N33/68 ,  B01J20/32

Abstract: The invention discloses an immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E, D, A, B, C) or protein Z or a functional variant thereof, wherein in at least one of the one or more mutated monomers, the asparagine or histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been deleted or substituted with a first amino acid residue which is not proline or asparagine and wherein, if the amino acid residue at position 57 is proline and the amino acid residue at position 28 is asparagine, then the amino acid residue at the position corresponding to H18 of the B domain of protein A or of protein Z is not serine, threonine or lysine.

公开(公告)号：US10112980B2

公开(公告)日：2018-10-30

申请号：US15598685

申请日：2017-05-18

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Mats Ander ,  Goran Bauren ,  Tomas Bjorkman ,  Gustav Rodrigo

IPC: C07K14/31 ,  C07K14/745 ,  C07K16/00 ,  C07K1/22 ,  B01D15/38 ,  B01J20/32 ,  B01J20/286

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamic acid residue at position 15 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.

公开(公告)号：US09896486B2

公开(公告)日：2018-02-20

申请号：US14903837

申请日：2014-07-08

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Tomas Bjorkman ,  Goran Bauren

IPC: C07K14/31 ,  C07K1/22 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32 ,  C07K14/745 ,  C07K16/00

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/745 ,  C07K16/00

Abstract: A polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A, as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.