公开(公告)号：US11650149B2

公开(公告)日：2023-05-16

申请号：US17040647

申请日：2019-03-26

Applicant: Georgia Tech Research Corporation

Inventor： Francisco Robles ,  Patrick Ledwig

IPC: G01N21/41 ,  G01N33/483 ,  G02B21/36 ,  G02B21/06 ,  G06K9/00 ,  G06V20/69

CPC classification number: G01N21/41 ,  G01N33/4833 ,  G02B21/06 ,  G02B21/365 ,  G06V20/693 ,  G06V20/698 ,  G01N2021/418 ,  G01N2201/061 ,  G01N2201/062 ,  G01N2201/0826

Abstract: Disclosed herein are systems and methods for imaging cells. Quantitative phase imaging uses variations in the index of refraction of a sample as a source of endogenous contrast, providing label-free information of sub-cellular structures and allowing for the reconstruction of valuable biophysical parameters, such as cell dry-mass at femtogram scales, mass transport, and sample thickness and fluctuations at nanometer scales. As a result, QPI has become a valuable tool in biology and medicine. However, QPI has suffered from the need for trans-illumination through relatively thin objects in order to gain access to the forward-scattered field, which carries crucial low spatial frequency information of a sample and avoid contributions from multiple scattered light or out-of-focus planes. The disclosed methods and systems can provide for reconstruction of QPI and corresponding analysis for imaging samples of cells in thick samples using an epi-illumination configuration.

公开(公告)号：US20240426749A1

公开(公告)日：2024-12-26

申请号：US18827944

申请日：2024-09-09

Applicant: Georgia Tech Research Corporation

Inventor： Francisco Robles ,  Patrick Ledwig

IPC: G01N21/41 ,  G01N33/483 ,  G02B21/06 ,  G02B21/36 ,  G06V20/69

Abstract: Systems and methods for imaging cells. Quantitative phase imaging uses variations in the index of refraction of a sample as a source of endogenous contrast, providing label-free information of sub-cellular structures and allowing for the reconstruction of valuable biophysical parameters, such as cell dry-mass at femtogram scales, mass transport, and sample thickness and fluctuations at nanometer scales. As a result, QPI has become a valuable tool in biology and medicine. However, QPI has suffered from the need for trans-illumination through relatively thin objects in order to gain access to the forward-scattered field, which carries crucial low spatial frequency information of a sample and avoid contributions from multiple scattered light or out-of-focus planes. The systems and methods can provide for reconstruction of QPI and corresponding analysis for imaging samples of cells in thick samples using an epi-illumination configuration.

公开(公告)号：US20230251192A1

公开(公告)日：2023-08-10

申请号：US18299408

申请日：2023-04-12

Applicant: Georgia Tech Research Corporation

Inventor： Francisco Robles ,  Patrick Ledwig

IPC: G01N21/41 ,  G01N33/483 ,  G02B21/06 ,  G02B21/36 ,  G06V20/69

CPC classification number: G01N21/41 ,  G01N33/4833 ,  G02B21/06 ,  G02B21/365 ,  G06V20/693 ,  G06V20/698 ,  G01N2021/418 ,  G01N2201/061 ,  G01N2201/062 ,  G01N2201/0826

Abstract: Systems and methods for imaging cells. Quantitative phase imaging uses variations in the index of refraction of a sample as a source of endogenous contrast, providing label-free information of sub-cellular structures and allowing for the reconstruction of valuable biophysical parameters, such as cell dry-mass at femtogram scales, mass transport, and sample thickness and fluctuations at nanometer scales. As a result, QPI has become a valuable tool in biology and medicine. However, QPI has suffered from the need for trans-illumination through relatively thin objects in order to gain access to the forward-scattered field, which carries crucial low spatial frequency information of a sample and avoid contributions from multiple scattered light or out-of-focus planes. The disclosed methods and systems can provide for reconstruction of QPI and corresponding analysis for imaging samples of cells in thick samples using an epi-illumination configuration.

公开(公告)号：US12140538B2

公开(公告)日：2024-11-12

申请号：US18299408

申请日：2023-04-12

Applicant: Georgia Tech Research Corporation

Inventor： Francisco Robles ,  Patrick Ledwig

IPC: G01N21/41 ,  G01N33/483 ,  G02B21/06 ,  G02B21/36 ,  G06V20/69

Abstract: Systems and methods for imaging cells. Quantitative phase imaging uses variations in the index of refraction of a sample as a source of endogenous contrast, providing label-free information of sub-cellular structures and allowing for the reconstruction of valuable biophysical parameters, such as cell dry-mass at femtogram scales, mass transport, and sample thickness and fluctuations at nanometer scales. As a result, QPI has become a valuable tool in biology and medicine. However, QPI has suffered from the need for trans-illumination through relatively thin objects in order to gain access to the forward-scattered field, which carries crucial low spatial frequency information of a sample and avoid contributions from multiple scattered light or out-of-focus planes. The disclosed methods and systems can provide for reconstruction of QPI and corresponding analysis for imaging samples of cells in thick samples using an epi-illumination configuration.

公开(公告)号：US20210025818A1

公开(公告)日：2021-01-28

申请号：US17040647

申请日：2019-03-26

Applicant: Georgia Tech Research Corporation

Inventor： Francisco Robles ,  Patrick Ledwig

IPC: G01N21/41 ,  G01N33/483 ,  G02B21/36 ,  G02B21/06 ,  G06K9/00

Abstract: Disclosed herein are systems and methods for imaging cells. Quantitative phase imaging uses variations in the index of refraction of a sample as a source of endogenous contrast, providing label-free information of sub-cellular structures and allowing for the reconstruction of valuable biophysical parameters, such as cell dry-mass at femtogram scales, mass transport, and sample thickness and fluctuations at nanometer scales. As a result, QPI has become a valuable tool in biology and medicine. However, QPI has suffered from the need for trans-illumination through relatively thin objects in order to gain access to the forward-scattered field, which carries crucial low spatial frequency information of a sample and avoid contributions from multiple scattered light or out-of-focus planes. The disclosed methods and systems can provide for reconstruction of QPI and corresponding analysis for imaging samples of cells in thick samples using an epi-illumination configuration.