公开(公告)号：US20170362634A1

公开(公告)日：2017-12-21

申请号：US15533087

申请日：2014-12-26

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Yuichiro OTA ,  Tomohiro SHOJI ,  Toru YOKOYAMA ,  Masatoshi NARAHARA

IPC: C12Q1/68 ,  G01N21/05 ,  G01N21/64

Abstract: The substrate 100 for use in the analysis of a nucleic acid according to the present invention has multiple analysis areas 12 which are partitioned on a substrate 10, and enables the measurement of the analysis areas 12 while interchanging the analysis areas 12 in turn, said substrate 100 being characterized in that each of the analysis areas 12 consists of an adsorption part 13 onto which a DNA fragment or a carrier having the DNA fragment carried thereon can be adsorbed and a non-adsorption part 14 which is a part outside of the adsorption part 13, and the non-adsorption part 14 has, formed on at least a part thereof, a marker part 15 that has a specified shape and helps to identify the positions of the analysis areas 12.

公开(公告)号：US20150337360A1

公开(公告)日：2015-11-26

申请号：US14652922

申请日：2013-12-04

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Toru YOKOYAMA ,  Motohiro YAMAZAKI

IPC: C12Q1/68 ,  G01N21/64 ,  G06F19/18 ,  G01N27/447

CPC classification number: C12Q1/68 ,  C12Q1/6827 ,  G01N21/6428 ,  G01N21/6456 ,  G01N27/447 ,  G01N27/44726 ,  G01N2021/6417 ,  G01N2021/6439 ,  G01N2021/6441 ,  G06F19/18 ,  C12Q2565/629 ,  C12Q2565/634

Abstract: A technique for performing spectral calibration simultaneously with electrophoresis of an actual sample to be analyzed, without performing electrophoresis using a special matrix standard which is time-consuming and costly, is provided. The device for genotypic analysis is characterized by obtaining reference fluorescence spectra using a size standard and an allelic ladder, which provide information concerning known DNA fragments used for electrophoresis of an actual sample, and is characterized by performing spectral calibration for a capillary in which the allelic ladder is not used by detecting a shift amount of the fluorescence spectra of the size standard and shifting the reference fluorescence spectra using the shift amount to determine fluorescence spectra.

Abstract translation: 提供了一种用于在不使用特别的基质标准进行电泳而不需要耗费时间和费用的电泳的情况下，与要分析的实际样品的电泳同时进行光谱校准的技术。 用于基因型分析的装置的特征在于使用大小标准和等位基因梯度获得参考荧光光谱，其提供关于用于实际样品的电泳的已知DNA片段的信息，其特征在于对毛细管进行光谱校准，其中等位基因 通过检测尺寸标准的荧光光谱的偏移量并使用移位量移动参考荧光光谱以确定荧光光谱，不使用梯子。

公开(公告)号：US20160109368A1

公开(公告)日：2016-04-21

申请号：US14890246

申请日：2014-05-09

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Michiru FUJIOKA ,  Motohiro YAMAZAKI ,  Toru YOKOYAMA

IPC: G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6486 ,  C12Q1/68 ,  G01N21/6408 ,  G01N2021/6415 ,  G01N2201/06113 ,  G01N2201/12 ,  G06F19/24

Abstract: Provided is a nucleic acid analyzer, which does not require manual processes by a highly trained operator such as a researcher and is easy to use, small-sized, capable of accepting multiple samples, and performs speedy analysis, and a nucleic acid analysis method using the analyzer. The analyzer and method perform detection in a plurality of exposure times, provide a program for determining a threshold for signal detection, and determine whether a faint signal peak is a false signal peak.

Abstract translation: 本发明提供一种核酸分析装置，其不需要由研究者等高度训练有素的操作者进行手动处理，易于使用，小型化，能够接受多个样品，并进行快速分析，并且使用核酸分析方法 分析仪。 分析器和方法在多个曝光时间中执行检测，提供用于确定信号检测阈值的程序，并且确定微弱信号峰值是否为假信号峰值。