公开(公告)号：US20190010443A9

公开(公告)日：2019-01-10

申请号：US14699659

申请日：2015-04-29

Applicant: Heliae Development LLC

Inventor： Eneko Ganuza Taberna ,  Jason D Licamele ,  Anna Lee Tonkovich ,  Michael V Bellefeuille ,  Cameron Wyatt ,  Thomas J Kulaga ,  Adriano Galvez III

IPC: C12N1/12 ,  C12Q3/00

Abstract: Methods of culturing mixotrophic microorganisms in a state of balanced gas composition are disclosed. Parameters of a culture of mixotrophic microorganisms may be controlled to reduce the requirements of externally supplied gases and optimize the production and consumption of gases within the culture by the phototrophic and heterotrophic metabolisms of the mixotrophic microorganisms.

公开(公告)号：US09856447B2

公开(公告)日：2018-01-02

申请号：US14805267

申请日：2015-07-21

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Anna Lee Tonkovich ,  Thomas Adame ,  Shan Qin ,  Eneko Ganuza Taberna

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/26 ,  C12M1/34 ,  C12M1/42 ,  C12M1/02 ,  C12M1/36

CPC classification number: C12M23/22 ,  C12M21/02 ,  C12M23/38 ,  C12M23/44 ,  C12M23/58 ,  C12M29/00 ,  C12M29/20 ,  C12M33/00 ,  C12M35/00 ,  C12M41/00 ,  C12M41/06 ,  C12M41/12 ,  C12M41/18 ,  C12M41/26 ,  C12M41/32 ,  C12M41/34 ,  C12M41/36 ,  C12M41/48

Abstract: Methods and apparatus for culturing microorganisms are described, including culturing in mixotrophic culture conditions. A bioreactor array with multiple culture vessels with independently controllable inputs is used to culture similar cultures of microorganisms in which at least one parameter differs from other culture vessels in the bioreactor array.

公开(公告)号：US09200236B2

公开(公告)日：2015-12-01

申请号：US13680961

申请日：2012-11-19

Applicant: Heliae Development, LLC

Inventor： Sandip Shinde ,  Aniket Kale ,  Tom Kulaga ,  Jason D. Licamele ,  Anna Lee Tonkovich

IPC: A61K8/36 ,  A61K8/97 ,  C11B3/00 ,  C07C1/22 ,  C12P7/64 ,  C12N1/12 ,  A23D9/00 ,  A23D9/02 ,  A23K1/16 ,  C11B1/10 ,  C11B3/12 ,  C11B7/00 ,  C11C3/02 ,  A23L1/30 ,  C11C3/00 ,  C10L1/02 ,  A61Q19/00

CPC classification number: C11B3/001 ,  A23D9/00 ,  A23D9/02 ,  A23K20/158 ,  A23L33/12 ,  A61K8/361 ,  A61K8/9706 ,  A61Q19/00 ,  C07C1/22 ,  C10G2300/1014 ,  C10L1/026 ,  C11B1/10 ,  C11B3/006 ,  C11B3/12 ,  C11B7/00 ,  C11C3/00 ,  C11C3/003 ,  C11C3/02 ,  C12N1/12 ,  C12P7/6409 ,  Y02E50/13 ,  Y02P30/20

Abstract: Disclosed here are compositions rich in omega-7 fatty acids, including palmitoleic acid, and products rich in omega-7 fatty acids derived from algal biomass. The algae and/or compositions rich in omega-7 fatty acids may be used in products or as ingredients of products. Methods and systems for increasing the production or concentration of omega-7 fatty acids, and isolating omega-7 fatty acids from algal biomass are also disclosed herein.

Abstract translation: 这里公开的是富含ω-7脂肪酸的组合物，包括棕榈油酸和富含来自藻类生物质的ω-7脂肪酸的产物。 富含ω-7脂肪酸的藻类和/或组合物可用于产品或产品的成分。 本文还公开了用于增加ω-7脂肪酸的产生或浓度以及从藻类生物质分离ω-7脂肪酸的方法和系统。

公开(公告)号：US20210079325A1

公开(公告)日：2021-03-18

申请号：US17098114

申请日：2020-11-13

Applicant: Heliae Development LLC

Inventor： Eneko Ganuza ,  Thomas Adame ,  Anna Lee Tonkovich ,  Adriano Galvez ,  Timothy Sullivan

IPC: C12M1/00 ,  C12M1/34 ,  C12M1/06 ,  C12M1/02

Abstract: Systems for culturing mixotrophic microorganisms on a large scale in thermally stable open systems are disclosed. Embodiments of the system include features such as lit portions, dark portions, organic carbon delivery systems, gas delivery systems, submersible thrusters for mixing, and turning vanes for guiding fluid flow. Multi-functional embodiments of the turning vane provide guidance for fluid flow and other functions such as heat exchange, nutrient delivery, gas delivery, organic carbon delivery, delivery of light, and parameter measurement by sensors.

公开(公告)号：US10240120B2

公开(公告)日：2019-03-26

申请号：US14699659

申请日：2015-04-29

Applicant: Heliae Development LLC

Inventor： Eneko Ganuza Taberna ,  Jason D Licamele ,  Anna Lee Tonkovich ,  Michael V Bellefeuille ,  Cameron Wyatt ,  Thomas J Kulaga ,  Adriano Galvez, III

IPC: C12N1/12 ,  C12Q3/00

Abstract: Methods of culturing mixotrophic microorganisms in a state of balanced gas composition are disclosed. Parameters of a culture of mixotrophic microorganisms may be controlled to reduce the requirements of externally supplied gases and optimize the production and consumption of gases within the culture by the phototrophic and heterotrophic metabolisms of the mixotrophic microorganisms.

公开(公告)号：US09416347B2

公开(公告)日：2016-08-16

申请号：US14872448

申请日：2015-10-01

Applicant: Heliae Development LLC

Inventor： Eneko Ganuza Taberna ,  Anna Lee Tonkovich

IPC: C12N1/12 ,  C12R1/89 ,  A61K36/02 ,  A01G33/00

CPC classification number: C12N1/12 ,  A01G33/00 ,  A61K36/02 ,  C12R1/89

Abstract: Methods of treating contamination, particularly bacterial contamination, in an open culture of microalgae with organic carbon are described herein. The methods comprise reducing the pH of a culture for a period of time and then raising the pH of the culture, wherein the culture comprises the presence of a first acid and the culture is contacted with a second acid to reduce the culture pH from a first pH value to a second pH value. The culture is maintained at the second pH value for at least 5 minutes before the culture pH is raised from the second pH value by contact with a base.

公开(公告)号：US20180105783A1

公开(公告)日：2018-04-19

申请号：US15821307

申请日：2017-11-22

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Anna Lee Tonkovich ,  Thomas Adame ,  Shan Qin ,  Eneko Ganuza Taberna ,  Michael LaMont ,  Steven Pflucker

IPC: C12M1/00 ,  C12M1/36 ,  C12M1/34 ,  C12M1/02 ,  C12M1/42 ,  C12M1/26 ,  C12M3/00

CPC classification number: C12M23/22 ,  C12M21/02 ,  C12M23/38 ,  C12M23/44 ,  C12M23/58 ,  C12M29/00 ,  C12M29/20 ,  C12M33/00 ,  C12M35/00 ,  C12M41/00 ,  C12M41/06 ,  C12M41/12 ,  C12M41/18 ,  C12M41/26 ,  C12M41/32 ,  C12M41/34 ,  C12M41/36 ,  C12M41/48

Abstract: Methods and apparatus for culturing microorganisms are described, including culturing in mixotrophic culture conditions. A bioreactor array with multiple culture vessels with independently controllable inputs is used to culture similar cultures of microorganisms in which at least one parameter differs from other culture vessels in the bioreactor array.

公开(公告)号：US20150322396A1

公开(公告)日：2015-11-12

申请号：US14805267

申请日：2015-07-21

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Anna Lee Tonkovich ,  Thomas Adame ,  Shan Qin ,  Eneko Ganuza

IPC: C12M1/00 ,  C12M1/02 ,  C12M1/34 ,  C12M1/42 ,  C12M3/00 ,  C12M1/26

CPC classification number: C12M23/22 ,  C12M21/02 ,  C12M23/38 ,  C12M23/44 ,  C12M23/58 ,  C12M29/00 ,  C12M29/20 ,  C12M33/00 ,  C12M35/00 ,  C12M41/00 ,  C12M41/06 ,  C12M41/12 ,  C12M41/18 ,  C12M41/26 ,  C12M41/32 ,  C12M41/34 ,  C12M41/36 ,  C12M41/48

Abstract: Methods and apparatus for culturing microorganisms are described, including culturing in mixotrophic culture conditions. A bioreactor array with multiple culture vessels with independently controllable inputs is used to culture similar cultures of microorganisms in which at least one parameter differs from other culture vessels in the bioreactor array.

Abstract translation: 描述了培养微生物的方法和装置，包括在培养条件下培养。 使用具有独立可控输入的多个培养容器的生物反应器阵列来培养相似的微生物培养物，其中至少一个参数与生物反应器阵列中的其它培养容器不同。

公开(公告)号：US20150240199A1

公开(公告)日：2015-08-27

申请号：US14699659

申请日：2015-04-29

Applicant: Heliae Development LLC

Inventor： Eneko Ganuza ,  Jason D Licamele ,  Anna Lee Tonkovich ,  Michael V Bellefeuille ,  Cameron Wyatt ,  Thomas J Kulaga ,  Adriano Galvez II

IPC: C12N1/12 ,  C12Q3/00

CPC classification number: C12N1/12 ,  C12Q3/00

Abstract: Methods of culturing mixotrophic microorganisms in a state of balanced gas composition are disclosed. Parameters of a culture of mixotrophic microorganisms may be controlled to reduce the requirements of externally supplied gases and optimize the production and consumption of gases within the culture by the phototrophic and heterotrophic metabolisms of the mixotrophic microorganisms.

Abstract translation: 公开了以平衡气体组成的状态培养混合营养微生物的方法。 可以控制混合营养微生物培养物的参数，以减少外部供应气体的需求，并通过混合营养微生物的光营养和异养代谢来优化培养物内的气体的生产和消耗。

公开(公告)号：US20160186129A1

公开(公告)日：2016-06-30

申请号：US14872448

申请日：2015-10-01

Applicant: Heliae Development LLC

Inventor： Eneko Ganuza ,  Anna Lee Tonkovich

IPC: C12N1/12

CPC classification number: C12N1/12 ,  A01G33/00 ,  A61K36/02 ,  C12R1/89

Abstract: Methods of treating contamination, particularly bacterial contamination, in an open culture of microalgae with organic carbon are described herein. The methods comprise reducing the pH of a culture for a period of time and then raising the pH of the culture, wherein the culture comprises the presence of a first acid and the culture is contacted with a second acid to reduce the culture pH from a first pH value to a second pH value. The culture is maintained at the second pH value for at least 5 minutes before the culture pH is raised from the second pH value by contact with a base.

Abstract translation: 本文描述了在有机碳的微藻的开放培养中处理污染物，特别是细菌污染物的方法。 所述方法包括将培养物的pH降低一段时间，然后提高培养物的pH，其中培养物包含第一种酸的存在，并使培养物与第二种酸接触，以将培养物pH从第一种 pH值达到第二个pH值。 将培养物保持在第二pH值至少5分钟，然后通过与碱接触培养pH从第二pH值升高。