公开(公告)号：US20190112658A1

公开(公告)日：2019-04-18

申请号：US16227732

申请日：2018-12-20

Applicant: IDEXX Laboratories, Inc.

Inventor： Christian Leutenegger ,  Michael John Angelichio

IPC: C12Q1/6876 ,  C12Q1/686 ,  C12Q1/6844

Abstract: The invention provides components and methods for polymerase chain reaction assays. The assays minimize both handling of material and time spent running samples. For example, a single internal positive control (IPC) polynucleotide pair can provide a means to ensure proper nucleic acid purification for both RNA and DNA test targets. Additionally, standard cycling conditions for all diagnostic tests allow the user to run both RNA and DNA targets side-by-side.

公开(公告)号：US10174373B2

公开(公告)日：2019-01-08

申请号：US14436739

申请日：2013-10-18

Applicant: IDEXX Laboratories, Inc.

Inventor： Christian Leutenegger ,  Michael John Angelichio

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6844 ,  C12Q1/686

Abstract: The invention provides components and methods for polymerase chain reaction assays. The assays minimize both handling of material and time spent running samples. For example, a single internal positive control (SPC) polynucleotide pair can provide a means to ensure proper nucleic acid purification for both RNA and DNA test targets. Additionally, standard cycling conditions for ali diagnostic tests allow the user to run both RNA and DNA targets side-by-side.

公开(公告)号：US11725242B2

公开(公告)日：2023-08-15

申请号：US16227732

申请日：2018-12-20

Applicant: IDEXX Laboratories, Inc.

Inventor： Christian Leutenegger ,  Michael John Angelichio

IPC: C12Q1/6876 ,  C12Q1/6844 ,  C12Q1/686

CPC classification number: C12Q1/6876 ,  C12Q1/686 ,  C12Q1/6844 ,  C12Q2600/166 ,  C12Q1/686 ,  C12Q2537/143 ,  C12Q2545/107 ,  C12Q2563/185

Abstract: The invention provides components and methods for polymerase chain reaction assays. The assays minimize both handling of material and time spent running samples. For example, a single internal positive control (IPC) polynucleotide pair can provide a means to ensure proper nucleic acid purification for both RNA and DNA test targets. Additionally, standard cycling conditions for all diagnostic tests allow the user to run both RNA and DNA targets side-by-side.

公开(公告)号：US20150275294A1

公开(公告)日：2015-10-01

申请号：US14436739

申请日：2013-10-18

Applicant: IDEXX LABORATORIES, INC.

Inventor： Christian Leutenegger ,  Michael John Angelichio

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2600/166 ,  C12Q2537/143 ,  C12Q2545/107 ,  C12Q2563/185

Abstract: The invention provides components and methods for polymerase chain reaction assays. The assays minimize both handling of material and time spent running samples. For example, a single internal positive control (SPC) polynucleotide pair can provide a means to ensure proper nucleic acid purification for both RNA and DNA test targets. Additionally, standard cycling conditions for ali diagnostic tests allow the user to run both RNA and DNA targets side-by-side.

Abstract translation: 本发明提供用于聚合酶链反应测定的组分和方法。 该测定最小化材料处理和运行样品花费的时间。 例如，单个内部阳性对照（SPC）多核苷酸对可以提供确保RNA和DNA测试目标的适当核酸纯化的手段。 此外，ali诊断测试的标准循环条件允许用户并排运行RNA和DNA靶标。