公开(公告)号：US20190338373A1

公开(公告)日：2019-11-07

申请号：US16401906

申请日：2019-05-02

Applicant: INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH

Inventor： Sandanaraj Selvaraj BRITTO ,  Punita BATHLA

IPC: C12Q1/6897 ,  C12Q1/37 ,  C12Q1/02

Abstract: Methods and materials for specific imaging of active enzyme in a live or intact cell are disclosed. The enzyme of interest tagged to reporter protein (donor) is exogenously expressed in a cell. The conversion of proenzyme to active enzyme (containing reporter protein) is achieved upon applying an appropriate stimulus to the target cells. The activated enzyme is labelled with an activity-based probe carrying a fluorophore (acceptor). The covalent labelling of active enzyme by the activity-based probe creates a FRET pair which provides the opportunity to exquisitely image the function of an “active enzyme”. This method is used for specific imaging of the function of active caspase-3,-7,-8,-9 and cathepsin-B and also for profiling of inhibitors of caspases and cathepsin B.

公开(公告)号：US20210163916A1

公开(公告)日：2021-06-03

申请号：US16771124

申请日：2018-12-08

Applicant: INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH

Inventor： Sandanaraj Selvaraj BRITTO ,  Pavankumar Janardhan BHANDARI ,  Mullapudi Mohan REDDY

IPC: C12N9/96 ,  C08G83/00 ,  A61K47/60 ,  C12N9/76 ,  C12N9/64

Abstract: The present invention discloses monodisperse protein-dendron conjugates that self-assemble to generation-dependent supramolecular protein assemblies of different size and surface charges. The invention further provides a process for synthesis of protein-dendron conjugates containing hydrophobic dendron of different generations.

公开(公告)号：US20190134212A1

公开(公告)日：2019-05-09

申请号：US16146891

申请日：2018-09-28

Applicant: INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH

Inventor： Sandanaraj Selvaraj BRITTO ,  Pavankumar Janardhan BHANDARI ,  Mullapudi Mohan REDDY

IPC: A61K47/64 ,  A61K38/48

Abstract: The present invention discloses stimuli-sensitive protein conjugate which can make supramolecular protein assemblies and methods for using the same. The present invention provides simple and rational process for construction of said stimuli-sensitive spherical protein assemblies through supramolecular chemical strategy.

公开(公告)号：US20170231260A1

公开(公告)日：2017-08-17

申请号：US15435055

申请日：2017-02-16

Applicant: INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH

Inventor： Sandanaraj Selvaraj BRITTO ,  Mullapudi Mohan REDDY ,  Pavankumar Janardhan BHANDARI ,  Kasuladevu Jagannadha RAO

IPC: A23L33/17 ,  A61K8/66 ,  A61Q17/00 ,  C12N9/50 ,  A61K51/08 ,  A61K49/14 ,  A61K49/00 ,  C07K1/14 ,  A61K39/00 ,  A61K9/48

CPC classification number: A23L33/17 ,  A23V2002/00 ,  A61K8/55 ,  A61K8/66 ,  A61K9/4825 ,  A61K2800/10 ,  A61Q19/00 ,  B82Y5/00 ,  C07K1/14 ,  C12N9/50 ,  C12Y304/21001 ,  C12Y304/21004 ,  C12Y304/21062 ,  C12Y304/21064

Abstract: The present invention discloses hydrophobin mimics of formula (I) comprising a protein head group, hydrophilic linker and hydrophobic tail and to a process for synthesis of library of hydrophobin mimics thereof. The hydrophobin mimics of the present invention self-assemble to form protein nanoparticles/nanocontainer either alone or in a specified chemical environment. The hydrophobin mimics (I) of the present invention find application in area of bio-nanotechnology.