公开(公告)号：US20160281149A1

公开(公告)日：2016-09-29

申请号：US14665904

申请日：2015-03-23

Applicant: InSilixa, Inc.

Inventor： Arjang Hassibi ,  Kshama Jirage ,  Arun Manickam ,  Kaveh Milaninia

IPC: C12Q1/68 ,  B01J19/00

CPC classification number: C12Q1/6837 ,  C12Q2527/107 ,  C12Q2565/101 ,  C12Q2565/607

Abstract: The present disclosure provides methods and devices for simultaneous identification of a plurality of target nucleic acid sequences in a single sample chamber that includes an addressable array of nucleic acid probes attached to a solid surface. Addressable signals can be generated and measured, in real-time, upon hybridization of target sequences at the individual probe locations within the array while the temperature of the system is varied. Such generated signals, as a function temperature, can then be used to compute the properties of nucleic acid hybridization at each addressable location which is ultimately utilized to estimate the sequence of the target nucleic acids. In particular, an integrated semiconductor biosensor array device can be used to measure the addressable signals.

Abstract translation: 本公开提供用于在单个样品室中同时鉴定多个靶核酸序列的方法和装置，其包括连接到固体表面的可寻址阵列的核酸探针。 当系统的温度变化时，可以在阵列内各个探针位置处的靶序列杂交时，实时产生和测量可寻址信号。 然后，作为功能温度的这样产生的信号可用于计算最终用于估计靶核酸序列的每个可寻址位置处的核酸杂交的性质。 特别地，可以使用集成半导体生物传感器阵列器件来测量可寻址信号。

公开(公告)号：US09708647B2

公开(公告)日：2017-07-18

申请号：US14665904

申请日：2015-03-23

Applicant: InSilixa, Inc.

Inventor： Arjang Hassibi ,  Kshama Jirage ,  Arun Manickam ,  Kaveh Milaninia

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q2527/107 ,  C12Q2565/101 ,  C12Q2565/607

Abstract: The present disclosure provides methods and devices for simultaneous identification of a plurality of target nucleic acid sequences in a single sample chamber that includes an addressable array of nucleic acid probes attached to a solid surface. Addressable signals can be generated and measured, in real-time, upon hybridization of target sequences at the individual probe locations within the array while the temperature of the system is varied. Such generated signals, as a function temperature, can then be used to compute the properties of nucleic acid hybridization at each addressable location which is ultimately utilized to estimate the sequence of the target nucleic acids. In particular, an integrated semiconductor biosensor array device can be used to measure the addressable signals.

公开(公告)号：US20240335832A1

公开(公告)日：2024-10-10

申请号：US18383385

申请日：2023-10-24

Applicant: InSilixa, Inc.

Inventor： Andrea Cuppoletti ,  Arjang Hassibi ,  Lei Pei ,  Yang Liu ,  Kshama Jirage ,  Arun Manickam

IPC: B01L3/00 ,  B01L7/00 ,  C07F7/08 ,  C23C16/02 ,  H01L27/146

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  C07F7/089 ,  C23C16/0272 ,  H01L27/14645 ,  B01L2200/0689 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2400/06

Abstract: The present disclosure provides methods and compositions for surface functionalization of solid substrates. The compositions include functionalized silanes and nucleic acid constructs which may react to immobilize the nucleic acid constructs on the surface on the solid substrate. The disclosure also provides methods for immobilization of silanes and nucleic acid constructs on the surface of the substrate.

公开(公告)号：US20230173483A1

公开(公告)日：2023-06-08

申请号：US17544776

申请日：2021-12-07

Applicant: InSilixa, Inc.

Inventor： Andrea Cuppoletti ,  Arjang Hassibi ,  Lei Pei ,  Yang Liu ,  Kshama Jirage ,  Arun Manickam

IPC: B01L3/00 ,  H01L27/146 ,  C23C16/02 ,  C07F7/08 ,  B01L7/00

CPC classification number: B01L3/502715 ,  H01L27/14645 ,  C23C16/0272 ,  C07F7/089 ,  B01L3/502738 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2400/06

Abstract: The present disclosure provides methods and compositions for surface functionalization of solid substrates. The compositions include functionalized silanes and nucleic acid constructs which may react to immobilize the nucleic acid constructs on the surface on the solid substrate. The disclosure also provides methods for immobilization of silanes and nucleic acid constructs on the surface of the substrate.

公开(公告)号：US11833503B2

公开(公告)日：2023-12-05

申请号：US17544776

申请日：2021-12-07

Applicant: InSilixa, Inc.

Inventor： Andrea Cuppoletti ,  Arjang Hassibi ,  Lei Pei ,  Yang Liu ,  Kshama Jirage ,  Arun Manickam

IPC: B01L3/00 ,  H01L27/146 ,  C23C16/02 ,  C07F7/08 ,  B01L7/00

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  C07F7/089 ,  C23C16/0272 ,  H01L27/14645 ,  B01L2200/0689 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2400/06

Abstract: The present disclosure provides methods and compositions for surface functionalization of solid substrates. The compositions include functionalized silanes and nucleic acid constructs which may react to immobilize the nucleic acid constructs on the surface on the solid substrate. The disclosure also provides methods for immobilization of silanes and nucleic acid constructs on the surface of the substrate.

公开(公告)号：US10174367B2

公开(公告)日：2019-01-08

申请号：US15291747

申请日：2016-10-12

Applicant: InSilixa, Inc.

Inventor： Arjang Hassibi ,  Kshama Jirage ,  Arun Manickam ,  Rituraj Singh

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6816 ,  C12Q1/6837

Abstract: The present disclosure provides methods, devices and systems that enable simultaneous multiplexing amplification reaction and real-time detection in a single reaction chamber.

公开(公告)号：US09499861B1

公开(公告)日：2016-11-22

申请号：US14850659

申请日：2015-09-10

Applicant: INSILIXA, INC.

Inventor： Arjang Hassibi ,  Kshama Jirage ,  Arun Manickam ,  Rituraj Singh

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q2565/101 ,  C12Q2565/519 ,  C12Q2565/607

Abstract: The present disclosure provides methods, devices and systems that enable simultaneous multiplexing amplification reaction and real-time detection in a single reaction chamber.

Abstract translation: 本公开提供了能够在单个反应室中同时进行多路复用放大反应和实时检测的方法，装置和系统。