公开(公告)号：US10533193B2

公开(公告)日：2020-01-14

申请号：US14914741

申请日：2014-08-05

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alex Van Eck Conradie ,  Adriana Leonora Botes

IPC: C12P5/02 ,  C12N1/21 ,  C12N9/88 ,  C12N9/12 ,  C12N9/16 ,  C12N9/10

Abstract: The document provides methods for biosynthesizing isobutene using one or more isolated enzymes such as one or more of a hydratase such as an enzyme classified under EC 4.2.1.- and a decarboxylating thioesterase, or using recombinant host cells expressing one or more such enzymes.

公开(公告)号：US10273518B2

公开(公告)日：2019-04-30

申请号：US14908659

申请日：2014-07-29

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Alex Van Eck Conradie ,  Adriana Leonora Botes

IPC: C12P19/32 ,  C12P7/40 ,  C12P7/26 ,  C12N9/10

Abstract: This document describes biochemical pathways that include the production of 3-oxopent-4-enoyl-CoA by condensation of acryloyl-CoA and acetyl-CoA using a β-ketothiolase with a SER-HIS-HIS catalytic triad. These pathways described herein rely on enzymes such as, inter alia, dehydrogenases, dehydratases and β-ketothiolases.

公开(公告)号：US20180023097A1

公开(公告)日：2018-01-25

申请号：US15493431

申请日：2017-04-21

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Paul S. Pearlman ,  Changlin Chen ,  Adriana Leonora Botes

IPC: C12P5/02 ,  C12N9/88 ,  C12P7/16 ,  C12N15/52 ,  C12N9/02 ,  C12P7/18

CPC classification number: C12P5/026 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12Y103/01035 ,  C12Y103/08 ,  C12Y114/11022 ,  C12Y401/01033 ,  C12Y402/01 ,  C12Y402/01127 ,  C12Y402/03027 ,  Y02E50/10 ,  Y02E50/343 ,  Y02P20/52

Abstract: Disclosed are methods for producing butadiene from one or more of several diverse feedstocks including bioderived feedstocks, renewable feedstocks, petrochemical feedstocks and natural gas.

公开(公告)号：US20160355844A1

公开(公告)日：2016-12-08

申请号：US15213830

申请日：2016-07-19

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Paul S. Pearlman ,  Changlin Chen ,  Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P5/02 ,  C12N15/52 ,  C12N9/88

CPC classification number: C12P5/026 ,  C12N9/001 ,  C12N9/0071 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12Y103/01035 ,  C12Y103/08 ,  C12Y114/11022 ,  C12Y401/01033 ,  C12Y402/01 ,  C12Y402/01127 ,  C12Y402/03027 ,  Y02E50/10 ,  Y02E50/343 ,  Y02P20/52

Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.

公开(公告)号：US20150079654A1

公开(公告)日：2015-03-19

申请号：US14334190

申请日：2014-07-17

Applicant: INVISTA North America S.a.r.l.

Inventor： Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P5/02

CPC classification number: C12P5/026 ,  C12Y402/01127 ,  Y02E50/343

Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as, inter alia, a decarboxylating thioesterase, cytochrome P450, or dehydratases for the final enzymatic step.

Abstract translation: 该文献描述了通过在丁二烯合成底物中形成两个乙烯基来生产丁二烯的生化途径。 本文描述的这些途径依赖于酶，例如脱羧硫酯酶，细胞色素P450或用于最终酶促步骤的脱水酶。

公开(公告)号：US20150004660A1

公开(公告)日：2015-01-01

申请号：US14490270

申请日：2014-09-18

Applicant: INVISTA North America S.a.r.l.

Inventor： Paul S. Pearlman ,  Changlin Chen ,  Adriana Leonora Botes

IPC: C12P13/00 ,  C12P5/02 ,  C12P17/10 ,  C12P7/44 ,  C12P7/04

CPC classification number: C12P13/005 ,  C07K14/195 ,  C07K14/37 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/78 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/42 ,  C12P7/44 ,  C12P7/46 ,  C12P13/001 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01004 ,  C12Y101/01157 ,  C12Y202/01006 ,  C12Y203/01009 ,  C12Y401/01005

Abstract: Embodiments of the invention relate to the enzymatic conversion of bioderived feedstocks to commercially valuable chemicals. The enzymatic conversions of the embodiments of the invention offer the potential for lower cost routes to these value-added chemicals. Some of the chemicals that are useful include nylon intermediates such as caprolactam, adipic acid, 1,6-hexamethylene diamine; butanediols such as 1,4-butanediol, 1,3-butanediol, and 2,3-butanediol; butanols such as 1-butanol, and 2-butanol; succinic acid, butadiene, isoprene, and 3-hydroxypropanoic acid.

Abstract translation: 本发明的实施方案涉及生物活性原料的酶转化为商业上有价值的化学品。 本发明实施方案的酶促转化提供了对这些增值化学品的较低成本路线的潜力。 一些有用的化学物质包括尼龙中间体如己内酰胺，己二酸，1,6-六亚甲基二胺; 丁二醇如1,4-丁二醇，1,3-丁二醇和2,3-丁二醇; 丁醇如1-丁醇和2-丁醇; 琥珀酸，丁二烯，异戊二烯和3-羟基丙酸。

公开(公告)号：US10538789B2

公开(公告)日：2020-01-21

申请号：US15808409

申请日：2017-11-09

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Mariusz Kamionka ,  Jan Modregger ,  Axel Niebisch ,  Alex Van Eck Conradie ,  Adriana Leonora Botes

IPC: C12P5/00 ,  C12N15/70 ,  C12P5/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N15/52

Abstract: This document describes biochemical pathways for producing isoprene by forming two vinyl groups in a central precursor produced from isobutyryl-CoA, 3-methyl-2-oxopentanoate, or 4-methyl-2-oxopentanoate as well as recombinant hosts for producing isoprene.

公开(公告)号：US20180057843A1

公开(公告)日：2018-03-01

申请号：US15674978

申请日：2017-08-11

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P5/02

CPC classification number: C12P5/026 ,  Y02E50/343

Abstract: The document provides methods for biosynthesizing isobutene using one or more isolated enzymes such as one or more of an enoyl-CoA dehydratase, a 2-hydroxyacyl-CoA dehydratase, an isovaleryl-CoA/acyl-CoA dehydrogenase and a mevalonate diphosphate decarboxylase, or using recombinant host cells expressing one or more such enzymes.

公开(公告)号：US20160355843A1

公开(公告)日：2016-12-08

申请号：US15211237

申请日：2016-07-15

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Paul S. Pearlman ,  Changlin Chen ,  Adriana Leonora Botes ,  Alex Van Eck Conradie

IPC: C12P5/02 ,  C08J5/00 ,  C07C11/167 ,  C08F136/06 ,  C12N15/52 ,  C12N9/88

CPC classification number: C12P5/026 ,  C07C11/167 ,  C08F136/06 ,  C08J5/00 ,  C08J2347/00 ,  C12N9/001 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12Y103/08 ,  C12Y401/01033 ,  C12Y402/01 ,  C12Y402/03027 ,  Y02E50/343 ,  Y02P20/52

Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.

公开(公告)号：US20180127788A1

公开(公告)日：2018-05-10

申请号：US15808409

申请日：2017-11-09

Applicant: INVISTA NORTH AMERICA S.A.R.L.

Inventor： Mariusz Kamionka ,  Jan Modregger ,  Axel Niebisch ,  Alex Van Eck Conradie ,  Adriana Leonora Botes

IPC: C12P5/00 ,  C12N15/70 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02

CPC classification number: C12P5/007 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12P5/026 ,  C12Y203/01041 ,  Y02E50/343

Abstract: This document describes biochemical pathways for producing isoprene by forming two vinyl groups in a central precursor produced from isobutyryl-CoA, 3-methyl-2-oxopentanoate, or 4-methyl-2-oxopentanoate as well as recombinant hosts for producing isoprene.