公开(公告)号：US11965167B2

公开(公告)日：2024-04-23

申请号：US16858443

申请日：2020-04-24

Applicant: Impossible Foods Inc.

Inventor： Biswajoy Roy Chaudhuri ,  Smita Shankar

IPC: C12N15/00 ,  C12N15/52

CPC classification number: C12N15/52 ,  C12Y203/01037

Abstract: This document relates to materials and methods for the production of protein. In one aspect, this document provides a first exogenous nucleic acid construct including a nucleotide sequence encoding an aminolevulinate synthase (ALAS) protein operably linked to a first promoter element, wherein the ALAS includes at least a first heme responsive motif (HRM), and wherein the ALAS includes a mutation in the first HRM, and a second exogenous nucleic acid construct comprising a nucleotide sequence encoding a heme-binding protein, wherein the second exogenous nucleic acid construct including a nucleotide sequence encoding the heme-binding protein is operably linked to the first promoter element or is operably linked to a second promoter element.

公开(公告)号：US20240218378A1

公开(公告)日：2024-07-04

申请号：US18609276

申请日：2024-03-19

Applicant: Impossible Foods Inc.

Inventor： Biswajoy Roy Chaudhuri ,  Smita Shankar

IPC: C12N15/52

CPC classification number: C12N15/52 ,  C12Y203/01037

Abstract: This document relates to materials and methods for the production of protein. In one aspect, this document provides a first exogenous nucleic acid construct including a nucleotide sequence encoding an aminolevulinate synthase (ALAS) protein operably linked to a first promoter element, wherein the ALAS includes at least a first heme responsive motif (HRM), and wherein the ALAS includes a mutation in the first HRM, and a second exogenous nucleic acid construct comprising a nucleotide sequence encoding a heme-binding protein, wherein the second exogenous nucleic acid construct including a nucleotide sequence encoding the heme-binding protein is operably linked to the first promoter element or is operably linked to a second promoter element.

公开(公告)号：US20230193338A1

公开(公告)日：2023-06-22

申请号：US18066890

申请日：2022-12-15

Applicant: Impossible Foods Inc.

Inventor： Biswajoy Roy Chaudhuri ,  Svetlana Balatskaya ,  Martin Andrew Hoyt

IPC: C12P21/00 ,  C12N15/81

CPC classification number: C12P21/00 ,  C12N15/815 ,  C12N2800/102

Abstract: Materials and methods that involve overexpression of a transcriptional activator such as retrograde regulation protein 1 (Rtg1) for increasing expression of one or more polypeptides.