公开(公告)号：US11592437B2

公开(公告)日：2023-02-28

申请号：US16802179

申请日：2020-02-26

Applicant: Indian Institute of Science

Inventor： Pallavi Dasgupta ,  Patnam Krishnaswamy ,  Vinay Kumar ,  Navakanta Bhat

IPC: G01N33/487 ,  G01N27/327 ,  G01N27/333 ,  G01N33/49 ,  G01N33/493

Abstract: Methods and devices for quantifying creatinine in a test sample are provided. The test sample is contacted with a sensing composition to obtain a product comprising a hydantoin-transition metal complex and ammonia. The sensing composition comprises creatinine deaminase and a transition metal salt. The creatinine deaminase enzymatically reacts with creatinine to provide the N-methyl hydantoin and ammonia. The N-methyl hydantoin forms the hydantoin-transition metal complex with the transition salt. A potential difference is applied to the product to measure a current signal provided by the hydantoin-transition metal complex. Concentration of N-methyl hydantoin is obtained based on the measured current signal using a calibration equation. The concentration of N-methyl hydantoin is correlated with concentration of creatinine to quantify the creatinine in the test sample.