公开(公告)号：US20220298541A1

公开(公告)日：2022-09-22

申请号：US17698397

申请日：2022-03-18

Applicant: Indian Institute of Science Education and Research

Inventor： Britto S. SANDANARAJ ,  Punita BATHLA ,  Abhijit DE ,  Aaiyas MUJAWAR

IPC: C12Q1/66 ,  G01N33/58 ,  G01N33/50

Abstract: The present disclosure relates to an assay for determining drug-target engagement in real-time. Specifically, the present disclosure provides a method for determining drug candidate-target interaction in real-time in living systems using Activity-based Reporter Gene Technology-Bioluminescence Resonance Energy Transfer (AbRGT-BRET) based assay. The said assay is useful for High Throughput Screening of compounds to a specific target.