公开(公告)号：US09146248B2

公开(公告)日：2015-09-29

申请号：US13802957

申请日：2013-03-14

Applicant: INTELLIGENT BIO-SYSTEMS, INC.

Inventor： Thomas Hagerott ,  Edmund W. Golaski

IPC: G01N35/10 ,  B01L3/00

CPC classification number: G01N35/1004 ,  B01L3/502707 ,  G01N35/085 ,  G01N35/10 ,  G01N35/1095 ,  Y10T29/49826

Abstract: A method for operating a nucleic acid sequencing instrument with movable flow cells. The method includes providing a flow cell having a flow path with an inlet port and an outlet port and filling the flow path, from the inlet port to the outlet port, with a first liquid reagent. The method also includes providing a station block having an inlet passage and an outlet passage, mounting the flow cell on the station block with the inlet port in fluid communication with the inlet passage and the outlet port in fluid communication with the outlet passage, introducing a gaseous bubble into the inlet port, conveying a second liquid reagent from the inlet passage into the inlet port to move the gaseous bubble through the flow path and into the outlet passage and fill the flow passage with the second liquid reagent, and removing the flow cell from the station block.

Abstract translation: 用于操作具有可移动流通池的核酸测序仪的方法。 该方法包括提供具有入口和出口的流路的流动池，并且从入口端口到出口端口用第一液体试剂填充流路。 该方法还包括提供具有入口通道和出口通道的站块，将流动池安装在站块上，入口与入口通道和出口端口流体连通，与出口通道流体连通，引入一个 将气泡进入入口，将第二液体试剂从入口通道输送到入口端口，以将气泡移动通过流动通道并进入出口通道并用第二液体试剂填充流动通道，并且移除流动池 从车站区块。

公开(公告)号：US08900810B2

公开(公告)日：2014-12-02

申请号：US13786895

申请日：2013-03-06

Applicant: Intelligent Bio Systems, Inc.

Inventor： Steven Gordon ,  Thomas Hagerott ,  Edmund Golaski ,  Jerzy Olejnik

IPC: C12Q1/68 ,  C12M3/00 ,  B01L3/00

CPC classification number: G01N27/44791 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0877 ,  B01L2400/0487 ,  C12Q1/6869 ,  G01N27/44726

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.

Abstract translation: 本发明提供了方法和组合物，包括但不限于使用例如通过合成方法的测序获得的数据来确定核苷酸序列中核酸的身份的算法，计算机可读介质，计算机程序，装置和系统。 为了提供更大的灵活性和效率，采用多个较小的流动池，每个具有相对小的要被成像的面积。