公开(公告)号：US20240076731A1

公开(公告)日：2024-03-07

申请号：US18178159

申请日：2023-03-03

Applicant: IsoPlexis Corporation

Inventor： Steven GORDON ,  Jerzy OLEJNIK

IPC: C12Q1/6869 ,  C07H19/00 ,  C12Q1/6874 ,  G01N21/05 ,  G01N21/64

CPC classification number: C12Q1/6869 ,  C07H19/00 ,  C12Q1/6874 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/645 ,  C12Q2535/101 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6471

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

公开(公告)号：US20220243266A1

公开(公告)日：2022-08-04

申请号：US17471932

申请日：2021-09-10

Applicant: IsoPlexis Corporation

Inventor： Steven GORDON ,  Daniel STETSON

IPC: C12Q1/6874 ,  B01L3/00 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6869

Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.

公开(公告)号：US20250052717A1

公开(公告)日：2025-02-13

申请号：US18663889

申请日：2024-05-14

Applicant: IsoPlexis Corporation

Inventor： Steven GORDON ,  Thomas HAGEROTT ,  Edmund GOLASKI ,  Jerzy OLEJNIK

IPC: G01N27/447 ,  B01L3/00 ,  C12Q1/6869

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.

公开(公告)号：US20240060128A1

公开(公告)日：2024-02-22

申请号：US18361434

申请日：2023-07-28

Applicant: IsoPlexis Corporation

Inventor： Steven GORDON ,  Jerzy OLEJNIK

IPC: C12Q1/6869 ,  C07H19/00 ,  C12Q1/6874 ,  G01N21/05 ,  G01N21/64

CPC classification number: C12Q1/6869 ,  C07H19/00 ,  C12Q1/6874 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/645 ,  C12Q2535/101 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6471

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

公开(公告)号：US20230407384A1

公开(公告)日：2023-12-21

申请号：US18325668

申请日：2023-05-30

Applicant: IsoPlexis Corporation

Inventor： Steven GORDON ,  Jerzy OLEJNIK

IPC: C12Q1/6869 ,  G01N21/64 ,  C12Q1/6874 ,  C07H19/00 ,  G01N21/05 ,  G01N21/03

CPC classification number: C12Q1/6869 ,  G01N21/6428 ,  C12Q1/6874 ,  G01N21/645 ,  C07H19/00 ,  G01N21/05 ,  G01N2201/062 ,  G01N2201/0627 ,  G01N2021/058 ,  G01N2021/6421 ,  G01N2021/0346 ,  G01N2201/12 ,  G01N2021/6417 ,  G01N2021/6471 ,  C12Q2535/101 ,  G01N2021/6441 ,  G01N2021/6439 ,  G01N2021/6419

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.