公开(公告)号：US20180044683A1

公开(公告)日：2018-02-15

申请号：US15552440

申请日：2016-02-19

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Kohsuke HONDA ,  Haruyuki ATOMI

IPC: C12N15/52 ,  C12N9/02 ,  C07H21/04 ,  C12N15/70 ,  C12N1/20 ,  C12N5/10

CPC classification number: C12N15/52 ,  C07H21/04 ,  C12N1/20 ,  C12N5/10 ,  C12N9/0036 ,  C12N15/09 ,  C12N15/70 ,  C12P19/32

Abstract: The present invention provides a method for synthesizing NAD+ or NADH in an enzyme reaction system requiring NAD+ or NADH so as to supplement NAD+ lost due to thermal decomposition in the enzyme reaction system, and a transformant set for coenzyme production used in the method. That is, the present invention relates to a method for producing a coenzyme including performing synthesis of NAD+ or NADH in an enzyme reaction system requiring NAD+ or NADH by adding one or more thermostable enzymes required for a reaction for synthesizing NAD+ from nicotinamide, to the enzyme reaction system, and a transformant set for coenzyme production including one or more transformants into which one or more genes encoding thermostable enzymes required for a reaction for synthesizing NAD+ or NADH from nicotinamide are introduced by using a non-thermostable microorganism as a host.