公开(公告)号：US20240060062A1

公开(公告)日：2024-02-22

申请号：US18489954

申请日：2023-10-19

Applicant: Jiangnan University

Inventor： Jie LONG ,  Ziying YE ,  Zhengyu JIN ,  Cheng Lu ,  Xingfei Li ,  Yaoqi TIAN ,  Yuxiang BAI ,  Xing ZHOU ,  Chao QIU ,  Long CHEN ,  Zhengjun XIE

IPC: C12N9/38 ,  C12N15/63

CPC classification number: C12N9/2468 ,  C12N15/63 ,  C12Y302/01081

Abstract: Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO.1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

公开(公告)号：US20240174770A1

公开(公告)日：2024-05-30

申请号：US18525796

申请日：2023-11-30

Applicant: Jiangnan University

Inventor： Xing ZHOU ,  Xiaoli LIANG ,  Zhengyu JIN ,  Ren WANG ,  Zhengjun XIE ,  Jianwei ZHAO ,  Jie LONG ,  Long CHEN ,  Chao QIU

IPC: C08B30/12 ,  A23L29/30

CPC classification number: C08B30/12 ,  A23L29/30

Abstract: A method for preparing V-type porous starch includes: mixing starch and a first ethanol solution in a reaction kettle, adding concentrated hydrochloric acid to the reaction kettle for acidity regulation, and controlling a temperature of a reaction system in the reaction kettle at 80-150° C.; cooling the reaction kettle, and adding a sodium hydroxide solution to the reaction system in the reaction kettle, until a pH of a mixture in the reaction kettle is neutral, to yield a starch solution; and centrifuging the starch solution, washing a collected precipitate with a second ethanol solution, leaching, drying, cooling, and pulverizing, to yield V-type porous starch.