公开(公告)号：US12286662B2

公开(公告)日：2025-04-29

申请号：US17763886

申请日：2020-08-05

Applicant: KANEKA CORPORATION

Inventor： Hisashi Arikawa ,  Shunsuke Sato ,  Yoshihiro Mouri

IPC: C12P7/625

Abstract: A polyhydroxyalkanoate-producing microorganism is cultured to obtain microbial bodies accumulating polyhydroxyalkanoate particles and having an average cell size of 2 μm or more. The microbial bodies are subjected to a heat treatment at a temperature higher than a temperature in the culturing to increase an average particle size of the polyhydroxyalkanoate particles in the microbial bodies. The resulting average particle size is equal to or greater than 1.8 μm and equal to or smaller than the average cell size. The microbial bodies subjected to the heat treatment can be disrupted to obtain a cell disruption solution. The PHA particles can be separated from an aqueous phase of the cell disruption solution.

公开(公告)号：US20190153486A1

公开(公告)日：2019-05-23

申请号：US16256780

申请日：2019-01-24

Applicant: KANEKA CORPORATION

Inventor： Hisashi Arikawa ,  Keiji Matsumoto

IPC: C12P7/62 ,  C08L67/04 ,  C08G63/06 ,  C12R1/01

Abstract: Provided are a transformant that produces a copolymerized PHA containing 3HH units in a higher composition proportion; and a method for producing a copolymerized PHA, using this transformant. The transformant is a transformant that produces a copolymerized PHA containing 3HH units, in which a gene encoding an enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is introduced into a prokaryotic microorganism having a PHA synthetase gene capable of synthesizing the copolymerized PHA containing the 3HH units. The method is a method for producing a copolymerized PHA containing 3HH units, which includes a step of culturing this transformant.

公开(公告)号：US10233468B2

公开(公告)日：2019-03-19

申请号：US14437672

申请日：2013-10-22

Applicant: KANEKA CORPORATION

Inventor： Hisashi Arikawa ,  Shunsuke Sato ,  Keiji Matsumoto

IPC: C12P7/62 ,  C12N15/74 ,  C12N9/18

Abstract: An object of the present invention is to provide a microorganism strain that accumulates a high molecular weight PHA, and a PHA production method using the microorganism. The present invention provides a method for producing a PHA copolymer, which includes culturing a microorganism, wherein at least a portion of either of the following genes (a) and (b) of the microorganism has been altered by substitution, deletion, insertion, and/or addition to reduce or eliminate the activity of a PHA degrading enzyme encoded by the gene: (a) a PHA degrading enzyme gene encoding the amino acid sequence of SEQ ID NO:2 in the sequence listing; and (b) a gene encoding a polypeptide having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 in the sequence listing and having PHA degrading enzyme activity.

公开(公告)号：US11453896B2

公开(公告)日：2022-09-27

申请号：US16927229

申请日：2020-07-13

Applicant: KANEKA CORPORATION

Inventor： Hisashi Arikawa ,  Shunsuke Sato

IPC: C12P7/625 ,  C12N9/10

Abstract: The present application provides: a transformed microorganism for producing a PHA copolymer containing 3HH monomer unit at a higher composition ratio, specifically, a transformed microorganism comprising a PHA synthase gene capable of synthesizing a PHA copolymer containing 3HH monomer unit and a gene encoding a protein having (R)-specific enoyl-CoA hydratase activity, characterized in that, in the transformed microorganism, the expression of a gene encoding at least one β-ketothiolase enzyme having thiolysis activity for β-keto-(C6) acyl-CoA (i.e., β-ketohexanoyl-CoA) is inhibited, thereby losing or reducing the enzyme activity; and a method for producing a PHA copolymer containing 3HH monomer unit, comprising a step of culturing the transformed microorganism.

公开(公告)号：US10829793B2

公开(公告)日：2020-11-10

申请号：US16256780

申请日：2019-01-24

Applicant: KANEKA CORPORATION

Inventor： Hisashi Arikawa ,  Keiji Matsumoto

IPC: C12N1/20 ,  C12P7/62 ,  C08G63/06 ,  C08L67/04 ,  C12R1/01 ,  C12N15/09

Abstract: Provided are a transformant that produces a copolymerized PHA containing 3HH units in a higher composition proportion; and a method for producing a copolymerized PHA, using this transformant. The transformant is a transformant that produces a copolymerized PHA containing 3HH units, in which a gene encoding an enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is introduced into a prokaryotic microorganism having a PHA synthetase gene capable of synthesizing the copolymerized PHA containing the 3HH units. The method is a method for producing a copolymerized PHA containing 3HH units, which includes a step of culturing this transformant.

公开(公告)号：US11225676B2

公开(公告)日：2022-01-18

申请号：US16670042

申请日：2019-10-31

Applicant: KANEKA CORPORATION

Inventor： Shingo Kobayashi ,  Rina Aoki ,  Tetsuya Minami ,  Hisashi Arikawa ,  Keiji Matsumoto

IPC: C12P7/62 ,  C12N9/10 ,  C08G63/06 ,  C12N9/88 ,  C12N15/09 ,  C08L101/16

Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.

公开(公告)号：US10519473B2

公开(公告)日：2019-12-31

申请号：US15129726

申请日：2015-03-27

Applicant: KANEKA CORPORATION

Inventor： Shingo Kobayashi ,  Rina Aoki ,  Tetsuya Minami ,  Hisashi Arikawa ,  Keiji Matsumoto

IPC: C12P7/62 ,  C12N9/10 ,  C12N9/88

Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.

公开(公告)号：US10072255B2

公开(公告)日：2018-09-11

申请号：US15115394

申请日：2015-01-30

Applicant: KANEKA CORPORATION

Inventor： Hisashi Arikawa ,  Shunsuke Sato ,  Keiji Matsumoto

IPC: C12P7/62 ,  C12N9/88 ,  C12N15/74

CPC classification number: C12N9/88 ,  C12N15/74 ,  C12P7/625 ,  C12Y402/01017 ,  C12Y402/01074

Abstract: This invention relates to a microorganism that produces a polyhydroxyalkanoate (PHA) copolymer with a regulated monomer composition ratio and comprises a (R)-specific enoyl-CoA hydratase gene in the genome DNA, wherein a nucleotide sequence upstream of the (R)-specific enoyl-CoA hydratase gene comprises a modification consisting of a substitution(s), a deletion(s), an insertion(s), and/or an addition(s) of one or a plurality of nucleotides so that the expression of the (R)-specific enoyl-CoA hydratase gene is regulated, and to a method for producing a PHA copolymer using the microorganism.