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公开(公告)号：US20210080333A1
公开(公告)日：2021-03-18
申请号：US17023003
申请日：2020-09-16
Inventor： Jieon LEE , Woo-keun KIM , Seokjoo YOON
IPC: G01K11/12 , C12Q1/6813 , C12Q1/6876 , C01B32/192 , G01N27/414
Abstract: The present invention relates to a composition for temperature sensors including graphene oxide, DNAzyme and PNA, a temperature sensing method and a kit using the same. In the present invention, when the DNAzyme/PNA duplex is dissociated at a certain temperature, graphene oxide adsorbs PNA with excellent selectivity and irreversibility, enabling recall of temperature, to permit delayed color development in the time of need. Also, the target temperature can be easily and quickly detected with the naked eye through the color change of the colorimetric reagent, and the thermosensor is technically convenient and easy to apply, so it can be used in various biological applications. Moreover, it can be used as a barcode (on/off) system using a combination of PNA probes with various lengths, and thus can be broadly applied to sensing a diverse range of temperatures.
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公开(公告)号：US20210324471A1
公开(公告)日：2021-10-21
申请号：US17006889
申请日：2020-08-30
Inventor： Jieon LEE , Woo-keun KIM , Seokjoo YOON
IPC: C12Q1/6883
Abstract: The present invention provides a complex of LNA probe and graphene oxide, and a nucleic acid detection method using the same. In the present invention, LNA-containing molecular beacon is conjugated through covalent bonding with graphene oxide, a single strand of the molecular beacon binds to a target nucleic acid to form a complex, and the complex is separated from graphene oxide to induce a fluorescence signal. The molecular beacon and graphene oxide can be covalently bonded to minimize non-specific signals, and a LNA-added molecular beacon is designed in a double strand to detect a very low concentration of target nucleic acid with high sensitivity, as well as a fluorescent signal, and the multiple target nucleic acids can be detected simultaneously through diversification of the fluorescent signal to enable easy and accurate detection of a nucleic acid biomarker whose specific expression level is specifically changed according to diseases and disease progression.
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公开(公告)号：US20210002695A1
公开(公告)日：2021-01-07
申请号：US16917135
申请日：2020-09-22
Inventor： Jieon LEE , Woo-keun KIM , Sangwoo LEE , Seokjoo YOON
IPC: C12Q1/682
Abstract: The present invention relates to a composition for detecting nucleic acid comprising duplex molecular beacon and graphene oxide and a colorimetric signal enhancement method of detecting nucleic acid using the same. According to the composition, kit and method for detecting nucleic acid of the present invention, a complex can be formed by adsorbing a single strand having a DNAzyme sequence dissociated from the conjugate of a duplex molecular beacon and a target nucleic acid to the graphene oxide surface, and separated, and a colorimetric signal amplified therefrom can be induced, so that a very low concentration of target nucleic acid can be detected with high efficiency and the target nucleic acid can be detected quickly and easily in seconds. Therefore, a new colorimetric target nucleic acid detection system capable of point of care testing (POCT) can be provided.
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