Candida antarctica lipase B mutant, and methods for making and using the same

    公开(公告)号:US10131889B1

    公开(公告)日:2018-11-20

    申请号:US15810007

    申请日:2017-11-11

    Inventor: Liming Liu Bin Yang

    Abstract: The present invention relates to the field of bioengineering. It provides a Candida antarctica lipase B mutant and its application. The mutant enzyme overcomes the limit of the parent enzyme that can exhibit high enantioselectivity towards (R)-3-TBDMSO glutaric acid methyl monoester only at temperatures below 5° C. The mutant enzyme successfully increased R-ee value at 5-70° C. The mutant D223V/A281S exhibits high R-ee value (>99%), high conversion rate (80%), and high space-time yield (107.54 g L−1 d−1). The present invention lays a foundation for industrial production of (R)-3-TBDMSO glutaric acid methyl monoester using a biosynthesis approach and provide insights into conformational dynamics-based enzyme design.

    Lower-power laser and arc hybrid welding method and device
    8.
    发明授权
    Lower-power laser and arc hybrid welding method and device 有权
    低功率激光和电弧复合焊接方法及装置

    公开(公告)号:US08969755B2

    公开(公告)日:2015-03-03

    申请号:US13518364

    申请日:2010-12-21

    Abstract: A low-power laser and arc hybrid welding method includes the steps of matching laser pulses with arc phases, and inducing compress arcs by the laser pulses. A laser peak pulse is triggered from half of the positive half-wave to half of the negative half-wave of alternating arc current. The sum of laser peak pulse width and laser basic pulse width is equal to the time width from a laser pulse triggered point to a negative half-wave end point of the alternating arc current. A welding device for carrying out the method is disclosed. An angle formed by the axis of a laser beam (1) and the vertical direction is in the range of −50°-50°. An angle formed by the axis of the laser beam (1) and the axis of an arc torch (2) is in the range of 20°-120°. Arc power density is adjusted minutely in the range of 103-105 by adjusting the alternating arc current, protrusion quantity of an electrode (3) and gas flow velocity of a nozzle. The method and the device can reduce the consumption and save the cost.

    Abstract translation: 低功率激光和电弧混合焊接方法包括以下步骤:将激光脉冲与电弧相匹配,并通过激光脉冲引发压缩弧。 激光峰值脉冲从正半波的一半触发到交变电弧电流的负半波的一半。 激光峰值脉冲宽度和激光基本脉冲宽度的总和等于从激光脉冲触发点到交变电弧电流的负半波终点的时间宽度。 公开了一种用于实施该方法的焊接装置。 由激光束(1)的轴线和垂直方向形成的角度在-50°-50°的范围内。 由激光束(1)的轴线和电弧割炬(2)的轴线形成的角度在20°-120°的范围内。 通过调节交流电弧电流,电极(3)的突出量和喷嘴的气体流速,可以在103-105的范围内微调电弧功率密度。 该方法和设备可以减少消耗并节省成本。

    Process for manufacturing super-high-count ramie fabric and the fabric
    10.
    发明授权
    Process for manufacturing super-high-count ramie fabric and the fabric 有权
    制造超高苎麻织物和织物的工艺

    公开(公告)号:US08375537B2

    公开(公告)日:2013-02-19

    申请号:US12601921

    申请日:2008-06-18

    CPC classification number: D03D15/06 D02G3/04 D02G3/406 D10B2201/01 D10B2201/08

    Abstract: The present invention relates to a process for manufacturing a ramie fabric and the fabric. The process comprising the following steps: blend spinning a high-count ramie fiber such as a ramie fiber of 2500Nm or higher with a water-soluble fiber as carrier to form a yarn; sizing the yarn at a low temperature; weaving the yarn to form a gray fabric; then removing the water-soluble fiber from the gray fabric by deweighting the gray fabric during a printing and dyeing finishing process to obtain a super-high-count ramie fabric with a ramie yarn fineness of 160Nm or higher.

    Abstract translation: 本发明涉及苎麻织物和织物的制造方法。 该方法包括以下步骤:将高倍数苎麻纤维如2500Nm或更高的苎麻纤维与水溶性纤维作为载体混纺以形成纱线; 在低温下定型纱线; 编织纱线形成灰色织物; 然后通过在印染整理过程中使灰色织物减重,从灰色织物中除去水溶性纤维,得到苎麻纱细度为160Nm以上的超高苎麻织物。

Patent Agency Ranking