公开(公告)号：US20230057482A1

公开(公告)日：2023-02-23

申请号：US17814674

申请日：2022-07-25

Applicant: Montana State University

Inventor： Blake A. WIEDENHEFT ,  Andrew SANTIAGO-FRANGOS ,  Anna A. NEMUDRAIA ,  Artem A. NEMUDRYI

IPC: C12Q1/70 ,  C12N9/22 ,  C12N15/11

Abstract: The disclosure relates to an engineered type III CRISPR-Cas system for sensitive and sequence specific detection of nucleic acid in a sample. For example, the engineered type III CRISPR-Cas system may be implemented as an assay for testing SARS-CoV-2 virus (or other target nucleic acid in the sample) that can be performed quickly, such as in one hour or less. Nucleic acid recognition by type III systems may trigger Cas10-mediated nuclease activity and/or polymerase activity, which may generate pyrophosphates, protons and cyclic oligonucleotides. The nuclease activity and/or the one or more products of the Cas10-polymerase are detected using colorimetric, visible fluorometric, and/or instrumented fluorometric detection.

公开(公告)号：US20230040061A1

公开(公告)日：2023-02-09

申请号：US17811391

申请日：2022-07-08

Applicant: Montana State University

Inventor： Blake A. WIEDENHEFT ,  Joseph E. NICHOLS ,  Anna A. NEMUDRAIA ,  Artem A. NEMUDRYI

IPC: C12N9/22 ,  C12N15/66 ,  C12N7/00 ,  C12N15/11

Abstract: CRISPR RNA-guided nucleases are routinely used for sequence-specific manipulation of DNA. While CRISPR-based DNA editing has become routine, analogous methods for editing RNA have yet to be established. Here we repurpose the type III-A CRISPR RNA-guided nuclease for sequence-specific cleavage of the SARS-CoV-2 genome. The type III cleavage reaction is performed in vitro using purified viral RNA, resulting in sequence-specific excision of 6, 12, 18 or 24 nucleotides. Ligation of the cleavage products is facilitated by a DNA splint that bridges the excision and RNA ligase is used to link the RNA products before transfection into mammalian cells. The SARS-CoV-2 RNA is infectious and standard plaque assays are used to recover viral clones. Collectively, this work demonstrates how type III CRISPR systems can be repurposed for sequence-specific editing of RNA viruses including SARS-CoV-2 and more generally for gene therapy.

公开(公告)号：US20250066752A1

公开(公告)日：2025-02-27

申请号：US18904927

申请日：2024-10-02

Applicant: Montana State University

Inventor： Blake A. WIEDENHEFT ,  Joseph E. NICHOLS ,  Anna A. NEMUDRAIA ,  Artem A. NEMUDRYI

IPC: C12N9/22 ,  C12N7/00 ,  C12N15/11 ,  C12N15/66

Abstract: CRISPR RNA-guided nucleases are routinely used for sequence-specific manipulation of DNA. While CRISPR-based DNA editing has become routine, analogous methods for editing RNA have yet to be established. Here we repurpose the type III-A CRISPR RNA-guided nuclease for sequence-specific cleavage of the SARS-COV-2 genome. The type III cleavage reaction is performed in vitro using purified viral RNA, resulting in sequence-specific excision of 6, 12, 18 or 24 nucleotides. Ligation of the cleavage products is facilitated by a DNA splint that bridges the excision and RNA ligase is used to link the RNA products before transfection into mammalian cells. The SARS-COV-2 RNA is infectious and standard plaque assays are used to recover viral clones. Collectively, this work demonstrates how type III CRISPR systems can be repurposed for sequence-specific editing of RNA viruses including SARS-COV-2 and more generally for gene therapy.

公开(公告)号：US20230323325A1

公开(公告)日：2023-10-12

申请号：US18299692

申请日：2023-04-12

Applicant: MONTANA STATE UNIVERSITY

Inventor： Blake A. WIEDENHEFT ,  Anna A. NEMUDRAIA ,  Artem A. NEMUDRYI

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/86

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/86 ,  C12N2310/20

Abstract: Engineered CRISPR proteins may be generated for localized anchoring to targeted cellular locations. Engineered CRISPR proteins may be generated by a lipidation motif with a CRISPR protein. The lipidation motif may be post-translationally modified to anchor the lipidation motifs and the fused CRISPR protein to a targeted cellular location, such as membranes of organelles associated with viral infections or other ailments. To account for possible additional amino acids that might affect the efficiency of post-translational modifications, linkers derived from C-terminal ends OAS1 (p46 isoform) and ZAP-L proteins may be used. Different fusion designs and/or different lipidation motifs may be used to target CRISPR proteins to specific and respective cellular locations. Targeted cellular localization of engineered CRISPR proteins may enable targeted therapies involving the engineered CRISPR proteins.

公开(公告)号：US20240182988A1

公开(公告)日：2024-06-06

申请号：US18331811

申请日：2023-06-08

Applicant: Montana State University

Inventor： Blake A. WIEDENHEFT ,  Andrew SANTIAGO-FRANGOS ,  Anna A. NEMUDRAIA ,  Artem A. NEMUDRYI

IPC: C12Q1/70 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6853

CPC classification number: C12Q1/701 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6853

Abstract: The disclosure relates to engineered systems and methods for detecting target nucleic acid in a sample, which may be a complex mixture. The systems and methods may improve sensitivity of target nucleic acid detection by enhancing signal generation. For example, signal generation may be enhanced through programmable capture and concentration of the target nucleic acid using an engineered type III CRISPR complex. Various ancillary nucleases such as Can1, Can2, and NucC are identified and may be used for detection. For example, binding of the engineered type III CRISPR complex may produce products that activate the identified ancillary nucleases. Different activators trigger changes in the substrate specificity of these nucleases. The activated nucleases may be used to detect programmatic detection of the target nucleic in the sample. The systems and methods are shown to detect viral RNA directly from nasopharyngeal swab samples.

公开(公告)号：US20230053573A1

公开(公告)日：2023-02-23

申请号：US17814097

申请日：2022-07-21

Applicant: Montana State University

Inventor： Blake A. WIEDENHEFT ,  Andrew SANTIAGO-FRANGOS ,  Anna A. NEMUDRAIA ,  Artem A. NEMUDRYI

IPC: C12Q1/70

Abstract: The disclosure relates to engineered systems and methods for detecting target nucleic acid in a sample, which may be a complex mixture. The systems and methods may improve sensitivity of target nucleic acid detection by enhancing signal generation. For example, signal generation may be enhanced through programmable capture and concentration of the target nucleic acid using an engineered type III CRISPR complex. Various ancillary nucleases such as Can1, Can2, and NucC are identified and may be used for detection. For example, binding of the engineered type III CRISPR complex may produce products that activate the identified ancillary nucleases. Different activators trigger changes in the substrate specificity of these nucleases. The activated nucleases may be used to detect programmatic detection of the target nucleic in the sample. The systems and methods are shown to detect viral RNA directly from nasopharyngeal swab samples.

公开(公告)号：US20210332446A1

公开(公告)日：2021-10-28

申请号：US17240858

申请日：2021-04-26

Applicant: MONTANA STATE UNIVERSITY

Inventor： Blake A. WIEDENHEFT ,  Andrew SANTIAGO-FRANGOS ,  Anna A. NEMUDRAIA ,  Artem A. NEMUDRYI

IPC: C12Q1/70 ,  C12N9/22 ,  C12N15/11

Abstract: The disclosure relates to an engineered type III CRISPR-Cas system for sensitive and sequence specific detection of nucleic acid in a sample. For example, the engineered type III CRISPR-Cas system may be implemented as an assay for testing SARS-CoV-2 virus (or other target nucleic acid in the sample) that can be performed quickly, such as in one hour or less. Nucleic acid recognition by type III systems may trigger Cas10-mediated nuclease activity and/or polymerase activity, which may generate pyrophosphates, protons and cyclic oligonucleotides. The nuclease activity and/or the one or more products of the Cas10-polymerase are detected using colorimetric, visible fluorometric, and/or instrumented fluorometric detection.