公开(公告)号：US20170190802A1

公开(公告)日：2017-07-06

申请号：US15410756

申请日：2017-01-19

Applicant: Massachusetts Institute of Technology

Inventor： Joseph M. Jacobson ,  David W. Mosley ,  Kie-Moon Sung

IPC: C08B37/16

CPC classification number: C08B37/0012 ,  B05D1/00 ,  C01B13/00 ,  C08B37/0015 ,  Y10S977/778 ,  Y10S977/882 ,  Y10T428/25 ,  Y10T428/2991

Abstract: Fabrication and arrangement of nanoparticles into one-dimensional linear chains is achieved by successive chemical reactions, each reaction adding one or more nanoparticles by building onto exposed, unprotected linker functionalities. Optionally, protecting groups may be used to control and organize growth. Nanoparticle spheres are functionalized in a controlled manner in order to enable covalent linkages. Functionalization of nanoparticles is accomplished by either ligand exchange or chemical modification of the terminal functional groups of the capping ligand. Nanoparticle chains are obtained by a variety of connectivity modes such as direct coupling, use of linker molecules, and use of linear polymeric templates. In particular, a versatile building block system is obtained through controlled monofunctionalization of nanoparticles.

公开(公告)号：US20170137858A1

公开(公告)日：2017-05-18

申请号：US15359560

申请日：2016-11-22

Applicant: Massachusetts Institute of Technology

Inventor： Peter A. Carr ,  Brian Y. Chow ,  Joseph M. Jacobson ,  David W. Mosley ,  Christopher Emig

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12P19/34 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6848 ,  C12Q2565/518 ,  C12Q2565/513 ,  C12Q2521/514

Abstract: In a method for generating an elongated nucleic acid molecule, a nucleic acid addition of a first nucleic acid molecule attached to a first 3′ or 5′ protecting group to a nucleic acid immobilized on a surface produces an intermediate-length immobilized nucleic acid. The first protecting group is dissociated from the first nucleic acid molecule. A second nucleic acid molecule that is attached to a second associated a 3′ or 5′ associated protecting group is added to the intermediate-length nucleic acid. The second associated protecting group is dissociated from the second nucleic acid molecule. A sequentially-extended elongated immobilized nucleic acid molecule having a desired sequence and length is produced by sequentially extending the intermediate-length immobilized nucleic acid by adding additional nucleic acid molecules with associated protecting groups to the intermediate-length nucleic acid and dissociating the associated protecting group after each addition.

公开(公告)号：US11268115B2

公开(公告)日：2022-03-08

申请号：US15359560

申请日：2016-11-22

Applicant: Massachusetts Institute of Technology

Inventor： Peter A. Carr ,  Brian Y. Chow ,  Joseph M. Jacobson ,  David W. Mosley ,  Christopher Emig

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6848 ,  C12Q1/6825 ,  C12Q1/6834

Abstract: In a method for generating an elongated nucleic acid molecule, a nucleic acid addition of a first nucleic acid molecule attached to a first 3′ or 5′ protecting group to a nucleic acid immobilized on a surface produces an intermediate-length immobilized nucleic acid. The first protecting group is dissociated from the first nucleic acid molecule. A second nucleic acid molecule that is attached to a second associated a 3′ or 5′ associated protecting group is added to the intermediate-length nucleic acid. The second associated protecting group is dissociated from the second nucleic acid molecule. A sequentially-extended elongated immobilized nucleic acid molecule having a desired sequence and length is produced by sequentially extending the intermediate-length immobilized nucleic acid by adding additional nucleic acid molecules with associated protecting groups to the intermediate-length nucleic acid and dissociating the associated protecting group after each addition.