公开(公告)号：US20150240273A1

公开(公告)日：2015-08-27

申请号：US14434651

申请日：2013-10-01

Applicant: Metabolix, Inc.

Inventor： Thomas Martin Ramseier ,  Jeffrey A. Bickmeier ,  William R. Farmer ,  Catherine Morse ,  Himani Chinnapen ,  Oliver P. Peoples ,  Yossef Shabtai

IPC: C12P7/62 ,  C08L67/04 ,  C08G63/06

CPC classification number: C12P7/625 ,  C08G63/06 ,  C08L67/04 ,  C08L2201/06

Abstract: A polyhydroxyalkanoate copolymer composition is provided. The composition comprises a plurality of polyhydroxyalkanoate copolymer molecules. The polyhydroxyalkanoate copolymer molecules (i) comprise 3-hydroxybutyrate monomers and 4-hydroxybutyrate monomers, (ii) have a monomeric molar percentage of 4-hydroxybutyrate monomers of 23.5 to 75%, and (iii) have a biobased content of ≧80%. Also provided is a method of making a polyhydroxyalkanoate copolymer composition. The method comprises culturing an organism in the presence of one or more carbon raw materials under conditions under which (a) the one or more carbon raw materials are converted to 3-hydroxybutyryl-CoA and 4-hydroxybutyryl-CoA and (b) the 3-hydroxybutyryl-CoA and the 4-hydroxybutyryl-CoA are polymerized to form the polyhydroxyalkanoate copolymer molecules, thereby forming the composition. The organism has been genetically engineered to comprise particular enzymatic activities, and to not comprise other particular enzymatic activities. The one or more carbon raw materials, taken together, have a biobased content of ≧80%.

Abstract translation: 提供聚羟基链烷酸酯共聚物组合物。 组合物包含多种聚羟基链烷酸酯共聚物分子。 聚羟基链烷酸酯共聚物分子（i）包含3-羟基丁酸酯单体和4-羟基丁酸酯单体，（ii）具有23.5-75％的4-羟基丁酸酯单体的单体摩尔百分数，和（iii）生物基含量≥80％。 还提供了制备聚羟基链烷酸酯共聚物组合物的方法。 该方法包括在（a）一种或多种碳原料转化为3-羟基丁酰辅酶A和4-羟基丁酰辅酶A的条件​​下，在一种或多种碳原料存在下培养生物体，和（b）3 聚羟基丁酰辅酶A和4-羟基丁酰辅酶A聚合形成聚羟基链烷酸酯共聚物分子，从而形成组合物。 生物体已被遗传工程化以包含特定的酶活性，并且不包含其它特定的酶活性。 一种或多种碳原料合在一起具有≥80％的生物基含量。