Abstract:
A three-dimensional (3D) reconstruction device and a method of flame spectra are provided. The device has a plurality of prism-mask shooting systems and a plurality of mirrors. The prism-type shooting systems are set up around a combustion area to be reconstructed and each is provided with two mirrors which are tilted on the left and right of an axis of the shooting system, so that data of a flame are reflected by the mirrors into the prism-mask shooting systems, thus realizing the real-time acquisition of hyperspectral data of the flame from multiple shooting angles. The reconstruction method includes following steps of acquiring, by a calibration cylinder, an area to be reconstructed, synchronously acquiring and preprocessing hyperspectral data of a flame, and finally reconstructing 3D spectral data of the flame by a 3D reconstruction algorithm of flame spectra.
Abstract:
A method for measuring a relative content of Gram-negative bacteria in an active sludge, the method including: 1) breaking zoogloeas in the active sludge; 2) isolating 2-keto-3-deoxyoctonic acid (KDO) from free bacteria and cell debris; 3) performing fluorescence labeling on the isolated KDO to obtain a fluorescent derivative; and 4) isolating the fluorescent derivative by high performance liquid chromatography (HPLC) and conducting quantitative measurement by fluorescence to obtain the relative content of the Gram-negative bacteria in the active sludge.
Abstract:
A high-speed and high-accuracy spectral video system has a filter module that filters optical signals in desired bands; a beam splitting module that splits the signal from the filter module into two identical beams entering an encoding aperture module and an RGB information acquisition module, respectively; a dispersion module disperses the optical signal and transmits the dispersed signal to a grayscale information acquisition module; a data reconstruction module aligns the signal from the grayscale information acquisition module to the signal from the RGB information acquisition module, denoises the signals, reconstructs a video by a bilateral filtering algorithm, and sends the reconstructed video to a display module for storage and display. A flame spectrum can be reconstructed using few sampling points to obtain broad-band spectral characteristics of the flame or using many sampling points to obtain high-accuracy spectral data.
Abstract:
A method for assessing bioaffinity of an organic biocarrier for wastewater treatment, the method including: 1) coating a matrix of an organic biocarrier on a standard chip to prepare a coated matrix chip; 2) preparing a testing water sample; 3) placing the coated matrix chip prepared in 1) in a quartz crystal microbalance (QCM), introducing the testing water sample prepared in 2) to the QCM, and measuring frequency data of the coated matrix chip under different overtones; and 4) fitting the frequency data of 3) using a Sauerbrey model to acquire variation of weights of an adlayer on a surface of the coated matrix chip with time; and comparing maximum fitted values of the weights of the adlayer on the surface of the coated matrix chip, to determine bioaffinity levels of the biocarriers to different testing water samples.
Abstract:
A method for analyzing water toxicity, the method including: exposure experiment, sample collection, transcriptome detection, metabolome detection, screening of differentially expressed genes, screening of differentially expressed metabolites, and identification of commonly changed biological pathways in both the transcriptome and the metabolome.