公开(公告)号：US09952196B2

公开(公告)日：2018-04-24

申请号：US14985986

申请日：2015-12-31

Applicant: NATIONAL CHUNG CHENG UNIVERSITY

Inventor： Shau-Chun Wang ,  Teh-Sheng Lai ,  Ting-Chou Chang

IPC: G01N33/487 ,  B01D61/44 ,  B01D61/46 ,  G01N21/25 ,  G01N27/447 ,  B01L3/00 ,  G01N1/40 ,  G01N21/552 ,  G01N33/68

CPC classification number: G01N33/487 ,  B01D61/44 ,  B01D61/46 ,  B01D2311/02 ,  B01D2311/2623 ,  B01D2311/2684 ,  B01L3/50 ,  G01N1/4005 ,  G01N21/251 ,  G01N21/554 ,  G01N27/44721 ,  G01N27/44791 ,  G01N33/6827 ,  G01N2001/4011 ,  G01N2201/0221 ,  G01N2201/12 ,  G01N2333/76

Abstract: A method for detecting albumin based on a colorimetric assay and a system thereof are disclosed. Gold nanoparticles are added into the sample preparing device having a sample without spectroscopic tags, wherein the sample without spectroscopic tags is formed as the alkaline solution to avoid the interference substances adhering on the gold nanoparticles. The gold nanoparticles are concentrated by using the microfluidic concentrator with the circular ion exchange membrane by applying an external electric field across two electrodes. The image of the concentrated gold nanoparticles is captured by the image capturing device for measuring the saturation intensities of the image, wherein there is a relation between the saturation intensities and the concentration of the albumin in the sample without spectroscopic tags. The concentration of the albumin of the sample without spectroscopic tags is obtained by the relation and the measured saturation intensities.

公开(公告)号：US09506861B2

公开(公告)日：2016-11-29

申请号：US14063763

申请日：2013-10-25

Applicant: NATIONAL CHUNG CHENG UNIVERSITY

Inventor： Shau-Chun Wang ,  Lai-Kwan Chau ,  Ting-Chou Chang ,  Chao-Ching Wu

IPC: G01N21/552 ,  G01N21/55 ,  G01N21/27 ,  G01N21/77

CPC classification number: G01N21/55 ,  G01N21/272 ,  G01N21/554 ,  G01N21/7703

Abstract: A method for obtaining the binding kinetic rate constants using fiber optic particle plasmon resonance (FOPPR) sensor, suitable for a test solution with two or more concentrations, which employs the following major steps: providing one FOPPR sensor instrument system, obtaining optical time-resolved signal intensities starting at the initial time to the steady state of the two or more regions, substituting the measured signal intensity values into the formula which is derived by using the pseudo-first order rate equation model. In addition, this method measures the temporal signal intensity evolution under static conditions as the samples are quickly loaded. As a result, unlike the conventional device where the sample is continuously infused, the method is able to measure the association and dissociation rate constants of which the upper bounds are not limited by the sample flow rate.

Abstract translation: 一种使用光纤粒子等离子体共振（FOPPR）传感器获得结合动力学速率常数的方法，适用于具有两个或多个浓度的测试溶液，其采用以下主要步骤：提供一个FOPPR传感器仪器系统，获得光学时间分辨 信号强度从初始时间开始到两个或更多个区域的稳定状态，将测量的信号强度值代入通过使用伪一阶速率方程模型导出的公式。 此外，该方法测量样品快速加载时在静态条件下的时间信号强度演化。 结果，与连续输入样品的常规装置不同，该方法能够测量上限的关联和解离速率常数不受样品流速的限制。