公开(公告)号：US11629351B2

公开(公告)日：2023-04-18

申请号：US17262507

申请日：2019-07-25

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Chikara Masuta ,  Tsuyoshi Inukai ,  Wataru Matsunaga ,  Reika Isoda ,  Takeshi Matsumura ,  Go Atsumi

IPC: C12N15/82

Abstract: Plants are created with desired traits by conveniently and rapidly inhibiting methylation of target DNA in plants, without using recombination technology. Scaffold RNA produced by transcription of target DNA is cleaved in an RNA-directed DNA methylation mechanism.

公开(公告)号：US20160002654A1

公开(公告)日：2016-01-07

申请号：US14769055

申请日：2014-02-20

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY

Inventor： Takeshi Matsumura ,  Noriho Fukuzawa ,  Noriko Itchouda ,  Chikara Masuta

IPC: C12N15/82

CPC classification number: C12N15/8257 ,  C12N15/8203 ,  C12N15/8206 ,  C12N15/8207

Abstract: A nucleic acid molecule for expressing a foreign gene in a plant, said nucleic acid molecule carrying, in this order, a right border (RB) sequence derived from the T-DNA sequence of an agrobacterium, an expression promoter sequence capable of functioning in the aforesaid plant, a sequence corresponding to the RNA2 genome of cucumber mosaic virus wherein a 2b protein-encoding gene has been partly or entirely substituted by the aforesaid foreign gene, and a left border (LB) sequence derived from the T-DNA sequence of an agrobacterium.

Abstract translation: 一种用于在植物中表达外源基因的核酸分子，所述核酸分子依次携带衍生自农杆菌的T-DNA序列的右边界（RB）序列，能够在农杆菌中起作用的表达启动子序列 上述植物，对应于黄瓜花叶病毒的RNA2基因组的序列，其中2b蛋白编码基因已被部分或完全被上述外源基因取代，以及衍生自上述外源基因的T-DNA序列的左边界（LB）序列 农杆菌。