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    Live Single-Cell Bioassay in Microdroplets
    1.
    发明申请
    Live Single-Cell Bioassay in Microdroplets 审中-公开

    公开(公告)号：US20180313844A1

    公开(公告)日：2018-11-01

    申请号：US15965841

    申请日：2018-04-27

    Applicant: Northeastern University

    Inventor： Tania KONRY ,  Saheli SARKAR ,  Pooja SABHACHANDANI

    IPC: G01N33/574 ,  B01L3/02 ,  B01L3/00 ,  G01N35/08 ,  G01N21/64 ,  G01N33/557

    Abstract: Bioassays are provided for detecting and analyzing responses from single cells and cell pairs suspended in micro-scale droplets (80-200 μm diameter) generated in microfluidic devices. Cell responses to various stimuli are analyzed. The stimuli are delivered by homotypic or heterotypic cells, small molecule drugs, and therapeutic agents including immunotherapeutics. The bioassays can be used to describe heterogeneity in any given cell population, and can be used in a clinical setting, such as profiling of patient-derived cells, designing personalized treatment strategies, and optimizing drug combinations for immunotherapy of tumors.

    Microfluidic Device for High-Throughput Screening of Tumor Cell Adhesion and Motility
    2.
    发明申请
    Microfluidic Device for High-Throughput Screening of Tumor Cell Adhesion and Motility 有权

    公开(公告)号：US20220212192A1

    公开(公告)日：2022-07-07

    申请号：US17613138

    申请日：2020-06-01

    Applicant: Northeastern University

    Inventor： Tania KONRY ,  Giovanni UGOLINI

    IPC: B01L3/00

    Abstract: Microfluidic devices and methods are provided for high-throughput generation, culturing, and analysis of cell spheroids, with subsequent isolation of selected cell spheroids for further analysis or isolation and expansion of cells from the spheroids. Any desired types of cells and matrices can be combined to form the cell spheroids and used to screen drugs and immunotherapy agents or methods, including in a personalized medicine format. Cell spheroids also can be cultivated and analyzed under hypoxic conditions. A particular advantage of the technology is the ability to isolate, enrich, and/or expand cells identified as having, or induced to have, desirable properties, such as immune cells that can be produced ex vivo and returned to the patient to combat a tumor or pathogen in vivo.

    Single Cell Isolation and Processing System With Reversible Well Shape
    3.
    发明申请
    Single Cell Isolation and Processing System With Reversible Well Shape 有权

    公开(公告)号：US20210260576A1

    公开(公告)日：2021-08-26

    申请号：US17182160

    申请日：2021-02-22

    Applicant: Northeastern University

    Inventor： Tania KONRY ,  Giovanni UGOLINI

    IPC: B01L3/00

    Abstract: The technology provides arrays of microwells with reversible shape for culture, analysis, and recovery of individual cells or groups of cells. The microwells are reversibly formed by vacuum-induced deflection of an elastomeric membrane into an array of microwell molds formed in a microwell substrate. The shape of each microwell or groups of microwells can be selectively altered by applying or releasing vacuum to individual microwells. The devices, systems, and methods utilizing the technology enable collection of individual cells for further study or therapeutic use without the need for micromanipulation.

    Exosome Detection in Microfluidic Droplets
    5.
    发明申请
    Exosome Detection in Microfluidic Droplets 有权

    公开(公告)号：US20210341485A1

    公开(公告)日：2021-11-04

    申请号：US17266671

    申请日：2019-08-08

    Applicant: Northeastern University

    Inventor： Saheli SARKAR ,  Tania KONRY

    IPC: G01N33/574 ,  C12Q1/6844 ,  G01N21/64

    Abstract: A platform technology for the detection and quantification of biomarkers in exosomes secreted by single cells uses a microfluidic system and provides methods of diagnosis and prognosis of cancer and other conditions using the biomarkers. Single cells can be obtained from a subject and characterized by their exosome population. The biomarkers can be detected at extremely low levels using rolling circle amplification and multiplex analysis to enhance specificity and sensitivity of the analysis.

    Single Cell Fluorescence in Situ Hybridization in Microfluidic Droplets
    6.
    发明申请
    Single Cell Fluorescence in Situ Hybridization in Microfluidic Droplets 有权

    公开(公告)号：US20210277455A1

    公开(公告)日：2021-09-09

    申请号：US16317308

    申请日：2017-07-12

    Applicant: Northeastern University

    Inventor： Saheli SARKAR ,  Tania KONRY

    IPC: C12Q1/6841 ,  G01N21/64 ,  B01L3/00

    Abstract: The invention provides a device, method, and system for high throughput detection of nucleic acid expression in individual cells. Cells are encapsulated in aqueous microdroplets which are merged with a biocompatible matrix, allowing on-chip fluorescence in situ hybridization on both adherent and non-adherent cells. The invention also provides multiplexed detection of nucleic acids, proteins, and cellular activity. The device and methods can be used to assess cellular interactions and to test the effects of antitumor agents.

    Single Cell Isolation and Processing System With Reversible Well Shape
    8.
    发明申请
    Single Cell Isolation and Processing System With Reversible Well Shape 有权

    公开(公告)号：US20250001420A1

    公开(公告)日：2025-01-02

    申请号：US18783022

    申请日：2024-07-24

    Applicant: Northeastern University

    Inventor： Tania KONRY ,  Giovanni UGOLINI

    IPC: B01L3/00

    Abstract: The technology provides arrays of microwells with reversible shape for culture, analysis, and recovery of individual cells or groups of cells. The microwells are reversibly formed by vacuum-induced deflection of an elastomeric membrane into an array of microwell molds formed in a microwell substrate. The shape of each microwell or groups of microwells can be selectively altered by applying or releasing vacuum to individual microwells. The devices, systems, and methods utilizing the technology enable collection of individual cells for further study or therapeutic use without the need for micromanipulation.

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