公开(公告)号：US20170058363A1

公开(公告)日：2017-03-02

申请号：US15303416

申请日：2015-04-10

Applicant: OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL CORPORATION

Inventor： Chuya Shinzato ,  Sutada Mungpakdee ,  Yuichi Nakajima

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: A method for identifying a subject of Acropora genus to an Acropora species comprises of detecting one or more microsatellite loci in the genome of the subject, wherein the microsatellite loci are selected from the group consisting of a microsatellite locus 8346m3 and others. A method for quantifying an index of level of chimerism of a coral colony restored by transplanting an Acropora first coral colony of an Acropora species into a second coral colony in need of restoration that belongs to the same species as the first coral colony comprises of identifying one or more microsatellite loci whose PCR fragment size, amplified with genomic DNA derived from the first coral colony as template, is different from the PCR fragment size amplified with genomic DNA derived from the second coral colony.

Abstract translation: 用于鉴定Acropora属的科目的方法包括检测受试者的基因组中的一个或多个微卫星位点，其中所述微卫星位点选自微卫星位点8346m3等。 通过将属于Acropora物种的Acropora第一珊瑚殖民地移植到属于与第一珊瑚群落相同的物种的需要恢复的第二个珊瑚群落来恢复珊瑚殖民地的嵌合体水平指数的方法包括鉴定一个 或更多的微卫星位点，其PCR片段大小用源自第一珊瑚群落的基因组DNA作为模板扩增，不同于用来自第二个珊瑚群落的基因组DNA扩增的PCR片段大小。