公开(公告)号：US09739698B2

公开(公告)日：2017-08-22

申请号：US15084246

申请日：2016-03-29

Applicant: OLYMPUS CORPORATION

Inventor： Tetsuya Tanabe ,  Seiji Kondo

IPC: G01N15/06 ,  G02B21/16 ,  G02B21/00 ,  G01N21/64 ,  G01N15/00

CPC classification number: G01N15/06 ,  G01N21/64 ,  G01N2015/0065 ,  G01N2015/0687 ,  G01N2015/0693 ,  G02B21/0032 ,  G02B21/0048 ,  G02B21/008 ,  G02B21/0084 ,  G02B21/16 ,  G02B2207/114

Abstract: In the scanning molecule counting method using optical measurement with a confocal or multiphoton microscope, there is provided a technique of computing a light-emitting particle concentration which changes with time and detecting a concentration change velocity or a reaction velocity. The inventive optical analysis technique of detecting light of light-emitting particles in a sample solution generates time series light intensity data of light from a light detection region detected with moving the position of the light detection region of the microscope in the sample solution; measures successively an interval of generation times of signals of the light-emitting particles detected in the time series light intensity data; and determines the concentration or concentration change velocity of the light-emitting particles, using the successively measured signal generation time intervals.

公开(公告)号：US20160209315A1

公开(公告)日：2016-07-21

申请号：US15084246

申请日：2016-03-29

Applicant: OLYMPUS CORPORATION

Inventor： Tetsuya Tanabe ,  Seiji Kondo

IPC: G01N15/06 ,  G02B21/16 ,  G02B21/00

CPC classification number: G01N15/06 ,  G01N21/64 ,  G01N2015/0065 ,  G01N2015/0687 ,  G01N2015/0693 ,  G02B21/0032 ,  G02B21/0048 ,  G02B21/008 ,  G02B21/0084 ,  G02B21/16 ,  G02B2207/114

Abstract: In the scanning molecule counting method using optical measurement with a confocal or multiphoton microscope, there is provided a technique of computing a light-emitting particle concentration which changes with time and detecting a concentration change velocity or a reaction velocity. The inventive optical analysis technique of detecting light of light-emitting particles in a sample solution generates time series light intensity data of light from a light detection region detected with moving the position of the light detection region of the microscope in the sample solution; measures successively an interval of generation times of signals of the light-emitting particles detected in the time series light intensity data; and determines the concentration or concentration change velocity of the light-emitting particles, using the successively measured signal generation time intervals.

Abstract translation: 在使用共焦或多光子显微镜的光学测量的扫描分子计数方法中，提供了计算随时间变化并检测浓度变化速度或反应速度的发光粒子浓度的技术。 本发明的检测样品溶液中的发光粒子的光的光学分析技术，通过在样品溶液中移动显微镜的光检测区域的位置，检测出来的光检测区域的光的时间序列的光强度数据; 连续地测量在时间序列光强数据中检测到的发光粒子的信号的产生次数的间隔; 并且使用连续测量的信号产生时间间隔来确定发光粒子的浓度或浓度变化速度。