公开(公告)号：US20160018391A1

公开(公告)日：2016-01-21

申请号：US14774002

申请日：2014-03-10

Applicant: SHARP KABUSHIKI KAISHA ,  OSAKA PREFECTURE UNIVERSITY PUBLIC CORPORATION

Inventor： Mugihei IKEMIZU ,  Shiho TOKONAMI ,  Hiroshi SHIIGI ,  Tsutomu NAGAOKA

IPC: G01N33/543 ,  C25D5/34 ,  C25D5/48 ,  G01N33/569

CPC classification number: G01N33/5438 ,  C12M41/36 ,  C12Q1/04 ,  C25D5/34 ,  C25D5/48 ,  G01N33/569 ,  G01N2600/00

Abstract: The present invention is a sensor for detecting a microorganism, which is provided with a detection unit equipped with a detection electrode and a polymer layer, wherein the polymer layer is arranged on the detection electrode and is provided with a template having a three-dimensional structure complementary to a three-dimensional structure of a microorganism to be detected. The sensor detects a microorganism on the basis of the captured state of the microorganism onto the template. The polymer layer is formed by a manufacturing method including a polymerization step of polymerizing a monomer in the presence of the microorganism to be detected to form a polymer layer having the microorganism incorporated therein on the detection electrode, and a disruption step of bringing at least a part of the microorganism incorporated in the polymer layer into contact with a solution containing a lytic enzyme to disrupt the microorganism.

Abstract translation: 本发明是一种用于检测微生物的传感器，其具有配备有检测电极和聚合物层的检测单元，其中聚合物层布置在检测电极上并且具有三维结构的模板 与待检测的微生物的三维结构互补。 传感器根据微生物的捕获状态将微生物检测到模板上。 聚合物层通过包括聚合步骤的方法形成，所述聚合步骤在待检测的微生物存在下聚合单体以在检测电极上形成其中包含微生物的聚合物层，以及至少使 引入到聚合物层中的微生物的一部分与含有裂解酶的溶液接触以破坏微生物。

公开(公告)号：US20170074760A1

公开(公告)日：2017-03-16

申请号：US15308895

申请日：2015-05-08

Applicant: OSAKA PREFECTURE UNIVERSITY PUBLIC CORPORATION ,  Sharp Kabushiki Kaisha

Inventor： Takuya IIDA ,  Shiho TOKONAMI ,  Ikuhiko NAKASE ,  Yushi NISHIMURA ,  Yasuyuki YAMAMOTO

IPC: G01N1/44 ,  C12M1/42

CPC classification number: G01N1/44 ,  B01J19/00 ,  B01J19/0046 ,  B01J2219/00441 ,  B01J2219/00468 ,  B01J2219/00711 ,  C12M1/26 ,  C12M1/34 ,  C12M35/02 ,  G01N15/00 ,  G01N15/14

Abstract: An assembling apparatus assembles beads different in particle size from each other. The assembling apparatus includes a substrate and a photothermal light source. The substrate is constructed to be able to hold a sample in which the beads are dispersed. The photothermal light source irradiates the substrate or the sample with laser beams to thereby produce a temperature difference in the sample.

Abstract translation: 组装装置将不同粒径的珠子组装在一起。 组装装置包括基板和光热光源。 衬底被构造成能够容纳其中分散有珠粒的样品。 光热光源用激光束照射基板或样品，从而在样品中产生温度差。

公开(公告)号：US20160123968A1

公开(公告)日：2016-05-05

申请号：US14894452

申请日：2014-05-30

Applicant: OSAKA PREFECTURE UNIVERSITY PUBLIC CORPORATION

Inventor： Takuya IIDA ,  Shiho TOKONAMI

IPC: G01N33/543 ,  G01N21/21 ,  G01N21/65 ,  G01N33/553 ,  G01N33/53

CPC classification number: G01N33/54346 ,  C12Q1/6834 ,  C12Q2563/155 ,  C12Q2565/628 ,  G01N21/21 ,  G01N21/554 ,  G01N21/658 ,  G01N33/5308 ,  G01N33/553 ,  G01N2201/061 ,  G01N2201/0683

Abstract: A detection device detects an analyte that may be contained in a specimen. The detection device includes a plurality of gold nanoparticles, an optical trapping light source, an illumination light source, an objective lens, an image pick-up device, and a computation unit. The plurality of gold nanoparticles are each modified with a probe DNA allowing the analyte to specifically adhere thereto. The optical trapping light source emits polarized light for assembling the plurality of gold nanoparticles together. The objective lens focuses and introduces the polarized light into a liquid containing a specimen and the plurality of gold nanoparticles. The image pick-up device receives light from the liquid. The computation unit detects an analyte based on a signal received from the image pick-up device.

Abstract translation: 检测装置检测可能包含在样本中的分析物。 检测装置包括多个金纳米颗粒，光学捕获光源，照明光源，物镜，图像拾取装置和计算单元。 多个金纳米颗粒各自用探针DNA修饰，允许分析物特异性地粘附于其上。 光捕获光源发射用于将多个金纳米颗粒组装在一起的偏振光。 物镜聚焦并将偏振光引入含有样品和多个金纳米颗粒的液体中。 摄像装置接收来自液体的光。 计算单元基于从图像拾取装置接收的信号检测分析物。

公开(公告)号：US20200182770A1

公开(公告)日：2020-06-11

申请号：US16301310

申请日：2017-05-11

Applicant: Osaka Prefecture University Public Corporation

Inventor： Shiho TOKONAMI ,  Takuya IIDA ,  Kazushi FUJIOKA

IPC: G01N15/08 ,  G01N1/44 ,  G02B21/32 ,  B01L3/00

Abstract: The purpose of the present invention is to collect a plurality of microscopic objects dispersed in a liquid by light irradiation, and also trap them. A collecting device for bacteria collects a plurality of bacteria dispersed in a sample liquid. The collecting device is provided with a laser beam source that emits laser beam and a honeycomb polymer film constituted so as to be able to hold the liquid. Walls prescribing pores for trapping the plurality of bacteria dispersed in the liquid are formed on the honeycomb polymer film, and also a thin film that includes a material for converting light from the laser beam source to heat is formed on the honeycomb polymer film. The thin film heats the liquid of the sample through the conversion of the laser beam from the laser beam source to heat, thereby causing a convection in the liquid.

公开(公告)号：US20170051242A1

公开(公告)日：2017-02-23

申请号：US15306515

申请日：2015-04-30

Applicant: OSAKA PREFECTURE UNIVERSITY PUBLIC CORPORATION ,  SHARP KABUSHIKI KAISHA

Inventor： Shiho TOKONAMI ,  Ikuhiko NAKASE

IPC: C12M1/12 ,  C08G73/06 ,  G01N33/574 ,  G01N33/566 ,  G01N33/50 ,  G01N33/543

CPC classification number: C12M25/02 ,  C08G73/0266 ,  C08G73/0611 ,  G01N33/5091 ,  G01N33/543 ,  G01N33/566 ,  G01N33/574 ,  G01N2600/00

Abstract: A polymer membrane for cancer cell detection having a surface provided with a mold having a three-dimensional structure complementary to a portion of a steric structure of a cancer cell to be detected; a method of producing the same; and a cancer cell detection device including the polymer membrane are provided. The polymer membrane for cancer cell detection can be obtained, for example, by a producing method including: polymerizing monomers in presence of cancer cells to be detected, to form a cancer cell-containing polymer membrane having the cancer cells incorporated therein; and removing at least part of the cancer cells incorporated in the cancer cell-containing polymer membrane.

Abstract translation: 一种用于癌细胞检测的聚合物膜，其具有设置有与要检测的癌细胞的空间结构的一部分互补的三维结构的模具的表面; 其制造方法; 并且提供包括聚合物膜的癌细胞检测装置。 用于癌细胞检测的聚合物膜可以通过例如包括在待检测的癌细胞的存在下聚合单体的方法获得，从而形成含有癌细胞的含癌细胞的聚合物膜; 以及去除掺入含癌细胞的聚合物膜中的至少部分癌细胞。